Acute Hypoxic Stress Affects Migration Machinery of Tissue O2-Adapted Adipose Stromal Cells
Joint Authors
Buravkova, Ludmila B.
Udartseva, Olga O.
Lobanova, Margarita V.
Andreeva, Elena R.
Buravkov, Sergey V.
Ogneva, I. V.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-28
Country of Publication
Egypt
No. of Pages
16
Abstract EN
The ability of mesenchymal stromal (stem) cells (MSCs) to be mobilised from their local depot towards sites of injury and to participate in tissue repair makes these cells promising candidates for cell therapy.
Physiological O2 tension in an MSC niche in vivo is about 4–7%.
However, most in vitro studies of MSC functional activity are performed at 20% O2.
Therefore, this study focused on the effects of short-term hypoxic stress (0.1% O2, 24 h) on adipose tissue-derived MSC motility at tissue-related O2 level.
No significant changes in integrin expression were detected after short-term hypoxic stress.
However, O2 deprivation provoked vimentin disassembly and actin polymerisation and increased cell stiffness.
In addition, hypoxic stress induced the downregulation of ACTR3, DSTN, MACF1, MID1, MYPT1, NCK1, ROCK1, TIAM1, and WASF1 expression, the products of which are known to be involved in leading edge formation and cell translocation.
These changes were accompanied by the attenuation of targeted and nontargeted migration of MSCs after short-term hypoxic exposure, as demonstrated in scratch and transwell migration assays.
These results indicate that acute hypoxic stress can modulate MSC function in their native milieu, preventing their mobilisation from sites of injury.
American Psychological Association (APA)
Udartseva, Olga O.& Lobanova, Margarita V.& Andreeva, Elena R.& Buravkov, Sergey V.& Ogneva, I. V.& Buravkova, Ludmila B.. 2016. Acute Hypoxic Stress Affects Migration Machinery of Tissue O2-Adapted Adipose Stromal Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1117087
Modern Language Association (MLA)
Udartseva, Olga O.…[et al.]. Acute Hypoxic Stress Affects Migration Machinery of Tissue O2-Adapted Adipose Stromal Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1117087
American Medical Association (AMA)
Udartseva, Olga O.& Lobanova, Margarita V.& Andreeva, Elena R.& Buravkov, Sergey V.& Ogneva, I. V.& Buravkova, Ludmila B.. Acute Hypoxic Stress Affects Migration Machinery of Tissue O2-Adapted Adipose Stromal Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1117087
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117087