Comparison of Osteogenesis between Adipose-Derived Mesenchymal Stem Cells and Their Sheets on Poly-ε-Caprolactoneβ-Tricalcium Phosphate Composite Scaffolds in Canine Bone Defects
Joint Authors
Kim, Yongsun
Lee, Seung Hoon
Kang, Byung-jae
Kim, Wan Hee
Yun, Hui-suk
Kweon, Oh-kyeong
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-16
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Multipotent mesenchymal stem cells (MSCs) and MSC sheets have effective potentials of bone regeneration.
Composite polymer/ceramic scaffolds such as poly-ε-caprolactone (PCL)/β-tricalcium phosphate (β-TCP) are widely used to repair large bone defects.
The present study investigated the in vitro osteogenic potential of canine adipose-derived MSCs (Ad-MSCs) and Ad-MSC sheets.
Composite PCL/β-TCP scaffolds seeded with Ad-MSCs or wrapped with osteogenic Ad-MSC sheets (OCS) were also fabricated and their osteogenic potential was assessed following transplantation into critical-sized bone defects in dogs.
The alkaline phosphatase (ALP) activity of osteogenic Ad-MSCs (O-MSCs) and OCS was significantly higher than that of undifferentiated Ad-MSCs (U-MSCs).
The ALP, runt-related transcription factor 2, osteopontin, and bone morphogenetic protein 7 mRNA levels were upregulated in O-MSCs and OCS as compared to U-MSCs.
In a segmental bone defect, the amount of newly formed bone was greater in PCL/β-TCP/OCS and PCL/β-TCP/O-MSCs/OCS than in the other groups.
The OCS exhibit strong osteogenic capacity, and OCS combined with a PCL/β-TCP composite scaffold stimulated new bone formation in a critical-sized bone defect.
These results suggest that the PCL/β-TCP/OCS composite has potential clinical applications in bone regeneration and can be used as an alternative treatment modality in bone tissue engineering.
American Psychological Association (APA)
Kim, Yongsun& Lee, Seung Hoon& Kang, Byung-jae& Kim, Wan Hee& Yun, Hui-suk& Kweon, Oh-kyeong. 2016. Comparison of Osteogenesis between Adipose-Derived Mesenchymal Stem Cells and Their Sheets on Poly-ε-Caprolactoneβ-Tricalcium Phosphate Composite Scaffolds in Canine Bone Defects. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1117262
Modern Language Association (MLA)
Kim, Yongsun…[et al.]. Comparison of Osteogenesis between Adipose-Derived Mesenchymal Stem Cells and Their Sheets on Poly-ε-Caprolactoneβ-Tricalcium Phosphate Composite Scaffolds in Canine Bone Defects. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1117262
American Medical Association (AMA)
Kim, Yongsun& Lee, Seung Hoon& Kang, Byung-jae& Kim, Wan Hee& Yun, Hui-suk& Kweon, Oh-kyeong. Comparison of Osteogenesis between Adipose-Derived Mesenchymal Stem Cells and Their Sheets on Poly-ε-Caprolactoneβ-Tricalcium Phosphate Composite Scaffolds in Canine Bone Defects. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1117262
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117262