Identification of Malignancy-Associated Changes in Histologically Normal Tumor-Adjacent Epithelium of Patients with HPV-Positive Oropharyngeal Cancer
Joint Authors
Jabalee, James
Carraro, Anita
Ng, Tony
Prisman, Eitan
Guillaud, Martial
Garnis, Cathie
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The incidence of HPV-positive oropharyngeal cancer (HPV+ OPC) is increasing, thus presenting new challenges for disease detection and management.
Noninvasive methods involving brush biopsies of diseased tissues were recently reported as insufficient for tumor detection in HPV+ OPC patients, likely due to differences between the site of tumor initiation at the base of involuted crypts and the site of brush biopsy at the crypt surface.
We hypothesized that histologically normal surface epithelial cells in the oropharynx contain changes in nuclear morphology that arise due to tumor proximity.
We analyzed the nuclear phenotype of matched tumor, tumor-adjacent normal, and contralateral normal tissues from biopsies of nine HPV+ OPC patients.
Measurements of 89 nuclear features were used to train a random forest-based classifier to discriminate between normal and tumor nuclei.
We then extracted voting scores from the trained classifier, which classify nuclei on a continuous scale from zero (“normal-like”) to one (“tumor-like”).
In each case, the average score of the adjacent normal nuclei was intermediate between the tumor and contralateral normal nuclei.
These results provide evidence for the existence of phenotypic changes in histologically normal, tumor-adjacent surface epithelial cells, which could be used as brush biopsy-based biomarkers for HPV+ OPC detection.
American Psychological Association (APA)
Jabalee, James& Carraro, Anita& Ng, Tony& Prisman, Eitan& Garnis, Cathie& Guillaud, Martial. 2018. Identification of Malignancy-Associated Changes in Histologically Normal Tumor-Adjacent Epithelium of Patients with HPV-Positive Oropharyngeal Cancer. Analytical Cellular Pathology،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1117424
Modern Language Association (MLA)
Jabalee, James…[et al.]. Identification of Malignancy-Associated Changes in Histologically Normal Tumor-Adjacent Epithelium of Patients with HPV-Positive Oropharyngeal Cancer. Analytical Cellular Pathology No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1117424
American Medical Association (AMA)
Jabalee, James& Carraro, Anita& Ng, Tony& Prisman, Eitan& Garnis, Cathie& Guillaud, Martial. Identification of Malignancy-Associated Changes in Histologically Normal Tumor-Adjacent Epithelium of Patients with HPV-Positive Oropharyngeal Cancer. Analytical Cellular Pathology. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1117424
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117424