Effects of Mechanical Stretch on Cell Proliferation and Matrix Formation of Mesenchymal Stem Cell and Anterior Cruciate Ligament Fibroblast
Joint Authors
Fan, Jiabing
Qu, Ling
Li, Hongguo
Liu, Xincheng
Zhu, Rui
Xue, Yingsen
Sun, Liguo
Fan, Hongbin
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-25
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Mesenchymal stem cells (MSCs) and fibroblasts are two major seed cells for ligament tissue engineering.
To understand the effects of mechanical stimulation on these cells and to develop effective approaches for cell therapy, it is necessary to investigate the biological effects of various mechanical loading conditions on cells.
In this study, fibroblasts and MSCs were tested and compared under a novel Uniflex/Bioflex culture system that might mimic mechanical strain in ligament tissue.
The cells were uniaxially or radially stretched with different strains (5%, 10%, and 15%) at 0.1, 0.5, and 1.0 Hz.
The cell proliferation and collagen production were compared to find the optimal parameters.
The results indicated that uniaxial stretch (15% at 0.5 Hz; 10% at 1.0 Hz) showed positive effects on fibroblast.
The uniaxial strains (5%, 10%, and 15%) at 0.5 Hz and 10% strain at 1.0 Hz were favorable for MSCs.
Radial strain did not have significant effect on fibroblast.
On the contrary, the radial strains (5%, 10%, and 15%) at 0.1 Hz had positive effects on MSCs.
This study suggested that fibroblasts and MSCs had their own appropriate mechanical stimulatory parameters.
These specific parameters potentially provide fundamental knowledge for future cell-based ligament regeneration.
American Psychological Association (APA)
Sun, Liguo& Qu, Ling& Zhu, Rui& Li, Hongguo& Xue, Yingsen& Liu, Xincheng…[et al.]. 2016. Effects of Mechanical Stretch on Cell Proliferation and Matrix Formation of Mesenchymal Stem Cell and Anterior Cruciate Ligament Fibroblast. Stem Cells International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1117482
Modern Language Association (MLA)
Sun, Liguo…[et al.]. Effects of Mechanical Stretch on Cell Proliferation and Matrix Formation of Mesenchymal Stem Cell and Anterior Cruciate Ligament Fibroblast. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1117482
American Medical Association (AMA)
Sun, Liguo& Qu, Ling& Zhu, Rui& Li, Hongguo& Xue, Yingsen& Liu, Xincheng…[et al.]. Effects of Mechanical Stretch on Cell Proliferation and Matrix Formation of Mesenchymal Stem Cell and Anterior Cruciate Ligament Fibroblast. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1117482
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1117482