Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats

Abstract EN

An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent.

Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats.

Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups.

Under general anesthesia, a full thickness skin wound (20 × 20 mm2) was created on the dorsum of each rat.

The defect in group I was kept as open wound and was taken as control.

In group II, the defect was repaired with commercially available collagen sheet (b-CS).

In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM).

Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process.

Significantly (P<0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21.

Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21.

These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats.