Assessment of Cytotoxic Activity of Rosemary (Rosmarinus officinalis L.), Turmeric (Curcuma longa L.), and Ginger (Zingiber officinale R.) Essential Oils in Cervical Cancer Cells (HeLa)
Joint Authors
Machinski Junior, Miguel
Santos, P. A. S. R.
Avanço, G. B.
Nerilo, S. B.
Marcelino, R. I. A.
Janeiro, V.
Valadares, M. C.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The objective of this study was to evaluate the cytotoxic activity of rosemary (REO, Rosmarinus officinalis L.), turmeric (CEO, Curcuma longa L.), and ginger (GEO, Zingiber officinale R.) essential oils in HeLa cells.
Cytotoxicity tests were performed in vitro, using tetrazolium (MTT) and neutral red assays for evaluation of antiproliferative activity by different mechanisms, trypan blue assay to assess cell viability and evaluation of cell morphology for Giemsa to observe the cell damage, and Annexin V to evaluate cell death by apoptosis.
CEO and GEO exhibited potent cytotoxic activity against HeLa cells.
IC50 obtained was 36.6 μg/mL for CEO and 129.9 μg/mL for GEO.
The morphology of HeLa cells showed condensation of chromatin, loss of cell membrane integrity with protrusions (blebs), and cell content leakage for cells treated with CEO and GEO, from the lowest concentrations studied, 32.81 μg/mL of CEO and 32.12 μg/mL of GEO.
The Annexin V assay revealed a profile of cell death by apoptosis for both CEO and GEO.
The results indicate cytotoxic activity in vitro for CEO and GEO, suggesting potential use as anticancer agents for cervical cancer cells.
American Psychological Association (APA)
Santos, P. A. S. R.& Avanço, G. B.& Nerilo, S. B.& Marcelino, R. I. A.& Janeiro, V.& Valadares, M. C.…[et al.]. 2016. Assessment of Cytotoxic Activity of Rosemary (Rosmarinus officinalis L.), Turmeric (Curcuma longa L.), and Ginger (Zingiber officinale R.) Essential Oils in Cervical Cancer Cells (HeLa). The Scientific World Journal،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1120641
Modern Language Association (MLA)
Santos, P. A. S. R.…[et al.]. Assessment of Cytotoxic Activity of Rosemary (Rosmarinus officinalis L.), Turmeric (Curcuma longa L.), and Ginger (Zingiber officinale R.) Essential Oils in Cervical Cancer Cells (HeLa). The Scientific World Journal No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1120641
American Medical Association (AMA)
Santos, P. A. S. R.& Avanço, G. B.& Nerilo, S. B.& Marcelino, R. I. A.& Janeiro, V.& Valadares, M. C.…[et al.]. Assessment of Cytotoxic Activity of Rosemary (Rosmarinus officinalis L.), Turmeric (Curcuma longa L.), and Ginger (Zingiber officinale R.) Essential Oils in Cervical Cancer Cells (HeLa). The Scientific World Journal. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1120641
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1120641
