Expression Profiling of Exosomal miRNAs Derived from the Peripheral Blood of Kidney Recipients with DGF Using High-Throughput Sequencing
Joint Authors
Wang, Junpeng
Li, Xin
Wu, Xiaoqiang
Wang, Zhiwei
Zhang, Chan
Cao, Guanghui
Yan, Tianzhong
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-12
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Delayed graft function (DGF) is one of the major obstacles for graft survival for kidney recipients.
It is profound to reduce the incidence of DGF for maintaining long-term graft survival.
However, the molecular regulation of DGF is still not adequately explained and the biomarkers for DGF are limited.
Exosomes are cell-derived membrane vesicles, contents of which are stable and could be delivered into recipient cells to exert their biological functions.
Consequently, exosome-derived proteomic and RNA signature profiles are often used to account for the molecular regulation of diseases or reflect the conditional state of their tissue as biomarkers.
Few researches have been done to demonstrate the function of exosomes associated with DGF.
In this study, high-throughput sequencing was used to explore the miRNA expression profiling of exosomes in the peripheral blood of kidney recipients with DGF.
We identified 52 known and 5 conserved exosomal miRNAs specifically expressed in recipients with DGF.
Three coexpressed miRNAs, hsa-miR-33a-5p_R-1, hsa-miR-98-5p, and hsa-miR-151a-5p, were observed to be significantly upregulated in kidney recipients with DGF.
Moreover, hsa-miR-151a-5p was positively correlated with the first-week serum CR, BUN, and UA levels of the kidney recipients after transplantation.
Furthermore, we also analyzed functions and signaling pathways of the three upregulated miRNAs target genes to uncover putative mechanism of how these exosomal miRNAs functioned in DGF.
Overall, these findings identified biomarker candidates for DGF and provided new insights into the important role of the exosomal miRNAs regulation in DGF.
American Psychological Association (APA)
Wang, Junpeng& Li, Xin& Wu, Xiaoqiang& Wang, Zhiwei& Zhang, Chan& Cao, Guanghui…[et al.]. 2019. Expression Profiling of Exosomal miRNAs Derived from the Peripheral Blood of Kidney Recipients with DGF Using High-Throughput Sequencing. BioMed Research International،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1123495
Modern Language Association (MLA)
Wang, Junpeng…[et al.]. Expression Profiling of Exosomal miRNAs Derived from the Peripheral Blood of Kidney Recipients with DGF Using High-Throughput Sequencing. BioMed Research International No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1123495
American Medical Association (AMA)
Wang, Junpeng& Li, Xin& Wu, Xiaoqiang& Wang, Zhiwei& Zhang, Chan& Cao, Guanghui…[et al.]. Expression Profiling of Exosomal miRNAs Derived from the Peripheral Blood of Kidney Recipients with DGF Using High-Throughput Sequencing. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1123495
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1123495