Transcriptome Sequencing to Detect the Potential Role of Long Noncoding RNAs in Salt-Sensitive Hypertensive Rats

Abstract EN

Backgrounds.

Long noncoding RNAs (lncRNAs) play an important role in various biological processes.

However, their functions in salt-sensitive hypertension are largely unknown.

In this study, the lncRNA-seq technique was employed to compare the expression profiles of lncRNAs and mRNAs in salt-sensitive hypertensive rats.

Methods.

Blood pressure, serum sodium, and urinary creatinine were texted in salt-sensitive and salt-insensitive rats fed with different salt concentrations.

High-throughput sequencing was used to detect the expression of lncRNAs and mRNA in the renal medulla of the two groups.

Results.

Blood pressure and urinary sodium/creatinine of high-salt diets of the sensitive group were significantly higher than that in the control group.

Serum sodium has no significant difference between the two groups in high-salt diets.

NONRATG007131.2 and NONRATG012674.2 were the most different lncRNAs in the high salt-sensitive group.

Correlation analysis reveals that Matn1, Serpinb12, Anxa8, and Hspa5 may play an important role in salt-sensitive hypertension.

Conclusion.

This study analyzed the difference in lncRNA and mRNA between salt-sensitive and salt-insensitive rats with different salt diets by high-throughput sequencing.

Salt sensitivity and salt concentration were two key factors for the induction of hypertension.

We found some potential genes that play an important role in salt-sensitive hypertension.