The Effect of Carbogen Breathing on 18F-FDG Uptake in Non-Small-Cell Lung Cancer
Joint Authors
Lin, Yu
Bao, Ying-Na
Huang, Cong-Xiu
Zhang, Ji-Hong
Yu, Zhi-Long
Tian, Ye
Wang, Xiang-Cheng
Cui, Yi-Tong
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-22
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
It has been reported that 18F-FDG uptake is higher in hypoxic cancer cells than in well-oxygenated cells.
We demonstrated that 18F-FDG uptake in lung cancer would be affected by high concentration oxygen breathing.
Methods.
Overnight fasted non-small-cell lung cancer A549 subcutaneous (s.c.) xenografts bearing mice (n = 10) underwent 18F-FDG micro-PET scans, animals breathed room air on day 1, and same animals breathed carbogen (95% O2 + 5% CO2) on the subsequent day.
In separated studies, autoradiography and immunohistochemical staining visualization of frozen section of A549 s.c.
tumors were applied, and to compare between carbogen-breathing mice and those with air breathing, a combination of 18F-FDG and hypoxia marker pimonidazole was injected 1 h before animal sacrifice, and 18F-FDG accumulation was compared with pimonidazole binding and glucose transporter 1 (GLUT-1) expression.
Results.
PET studies revealed that tumor 18F-FDG uptake was significantly decreased in carbogen-breathing mice than those with air breathing (P<0.05).
Ex vivo studies confirmed that carbogen breathing significantly decreased hypoxic fraction detected by pimonidazole staining, referring to GLUT-1 expression, and significantly decreased 18F-FDG accumulation in tumors.
Conclusions.
High concentration of O2 breathing during 18F-FDG uptake phase significantly decreases 18F-FDG uptake in non-small-cell lung cancer A549 xenografts growing in mice.
American Psychological Association (APA)
Lin, Yu& Bao, Ying-Na& Huang, Cong-Xiu& Zhang, Ji-Hong& Yu, Zhi-Long& Tian, Ye…[et al.]. 2019. The Effect of Carbogen Breathing on 18F-FDG Uptake in Non-Small-Cell Lung Cancer. BioMed Research International،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1124171
Modern Language Association (MLA)
Lin, Yu…[et al.]. The Effect of Carbogen Breathing on 18F-FDG Uptake in Non-Small-Cell Lung Cancer. BioMed Research International No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1124171
American Medical Association (AMA)
Lin, Yu& Bao, Ying-Na& Huang, Cong-Xiu& Zhang, Ji-Hong& Yu, Zhi-Long& Tian, Ye…[et al.]. The Effect of Carbogen Breathing on 18F-FDG Uptake in Non-Small-Cell Lung Cancer. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1124171
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124171