A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP
Joint Authors
Du, Lijuan
Miao, Yong
Li, Xin
Shi, Panli
Hu, Zhi-Qi
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-13
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Platelet rich plasma (PRP) is a concentrate of autologous platelets which contain enrichment growth factors (GFs).
However, the addition of exogenous anticoagulant and procoagulant may result in clinical side effects and raise the price of PRP.
Herein, we report a novel method named temperature controlled PRP (t-PRP), in which exogenous additives are dispensable in the preparation and activation process.
Human blood samples were processed by a two-step centrifugation process under hypothermic conditions (4°C) to obtain t-PRP and rewarming up to 37°C to activate t-PRP.
Contemporary PRP (c-PRP) was processed as the control.
t-PRP showed a physiological pH value between 7.46 and 7.48 and up to 6.58 ± 0.45-fold significantly higher platelet concentration than that of whole blood compared with c-PRP (4.06-fold) in the preparation process.
Meanwhile, t-PRP also maintained a stable GF level between plasma and PRP.
After activation, t-PRP demonstrated natural fiber scaffolding, which trapped more platelet and GFs, and exhibited a slow release and degradation rate of GFs.
In addition, t-PRP exhibited the function of promoting wound healing.
t-PRP is a novel and convenient method for the preparation and activation of PRP without any additives.
Compared to c-PRP, t-PRP reflects more physiologic characteristics while maintaining high quality.
American Psychological Association (APA)
Du, Lijuan& Miao, Yong& Li, Xin& Shi, Panli& Hu, Zhi-Qi. 2018. A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP. BioMed Research International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1124627
Modern Language Association (MLA)
Du, Lijuan…[et al.]. A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP. BioMed Research International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1124627
American Medical Association (AMA)
Du, Lijuan& Miao, Yong& Li, Xin& Shi, Panli& Hu, Zhi-Qi. A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1124627
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1124627