Fermentation Conditions and Media Optimization for Isocitric Acid Production from Ethanol by Yarrowia lipolytica
Joint Authors
Kamzolova, Svetlana V.
Shamin, Roman V.
Stepanova, Nadezda N.
Morgunov, Grigorii I.
Lunina, Julia N.
Allayarov, Ramil K.
Samoilenko, Vladimir A.
Morgunov, Igor G.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Isocitric acid exists in the form of four stereoisomers, of which only the threo-Ds-form (ICA) is a natural active compound, an intermediate of Krebs cycle, and suitable for nutritional and pharmaceutical use.
In this paper, we propose a method for ICA production from ethanol by yeast Yarrowia lipolytica.
The effects of temperature, pH of the medium, and aeration on the growth of the producer Y.
lipolytica VKM Y-2373 and synthesis of ICA were studied.
An optimal fermentation regime, which ensures a good growth of the producer and directed synthesis of the target product, was determined.
The producer is advised to carry out cultivation at 29°C and various pH of the medium and the oxygen concentration (pH 5 and pO2 20–25% (of saturation) during the growth period and pH 6 and pO2 50–55% (of saturation) during the acid formation) on a nutrient medium containing an increased content of zinc (0.6 mg/L), iron (1.2 mg/L), and 30 mM itaconic acid (inhibitor of isocitrate lyase—the key enzyme of ICA metabolism) should also be introduced into the nutrition medium.
Such fermentation production mode provides 90.5 g/L ICA with process selectivity of 80%, mass yield (YICA) of 0.77 g/g, and energy yield (ηICA) of 0.278 g/g.
American Psychological Association (APA)
Kamzolova, Svetlana V.& Shamin, Roman V.& Stepanova, Nadezda N.& Morgunov, Grigorii I.& Lunina, Julia N.& Allayarov, Ramil K.…[et al.]. 2018. Fermentation Conditions and Media Optimization for Isocitric Acid Production from Ethanol by Yarrowia lipolytica. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1125101
Modern Language Association (MLA)
Kamzolova, Svetlana V.…[et al.]. Fermentation Conditions and Media Optimization for Isocitric Acid Production from Ethanol by Yarrowia lipolytica. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1125101
American Medical Association (AMA)
Kamzolova, Svetlana V.& Shamin, Roman V.& Stepanova, Nadezda N.& Morgunov, Grigorii I.& Lunina, Julia N.& Allayarov, Ramil K.…[et al.]. Fermentation Conditions and Media Optimization for Isocitric Acid Production from Ethanol by Yarrowia lipolytica. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1125101
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125101