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Evaluation of the Candida sp. 99-125 Lipase Positional Selectivity for 1,3-Diolein Synthesis
Joint Authors
Wang, Zhaoyu
Bi, Yanhong
Zhao, Xiangjie
Duan, Zhangqun
Zhang, Wenjing
Xu, Lei
Zhao, Xiaojuan
Yang, Jiali
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
In this study, comparative experiments were carried out to investigate the positional selectivity of Candida sp.
99–125 lipase in preparing 1,3-diolein by using medium engineering strategy.
The results indicated that the diolein yield was markedly enhanced from 56.5% to 86.7% with increasing logP values of the solvents, while the selectivity of the examined lipase for the sn-1 over the sn-2 hydroxyl of glycerol was decreased, thus leading to a reduced 1,3-diolein to 1,2-diolein ratio.
To evaluate the possibility of industrial enzymatic production of 1,3-diolein, larger-scale experiments were assessed.
After being used repeatedly for eight batches, the diolein content reached 95.1%, while the 1,3-diolein to 1,2-diolein ratio was 7:1 following purification.
Results of the kg level experiments significantly demonstrated the practicability of the enzymatic process and the efficiency of the purification strategy for the product.
American Psychological Association (APA)
Bi, Yanhong& Duan, Zhangqun& Zhang, Wenjing& Xu, Lei& Wang, Zhaoyu& Zhao, Xiaojuan…[et al.]. 2019. Evaluation of the Candida sp. 99-125 Lipase Positional Selectivity for 1,3-Diolein Synthesis. BioMed Research International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1125270
Modern Language Association (MLA)
Bi, Yanhong…[et al.]. Evaluation of the Candida sp. 99-125 Lipase Positional Selectivity for 1,3-Diolein Synthesis. BioMed Research International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1125270
American Medical Association (AMA)
Bi, Yanhong& Duan, Zhangqun& Zhang, Wenjing& Xu, Lei& Wang, Zhaoyu& Zhao, Xiaojuan…[et al.]. Evaluation of the Candida sp. 99-125 Lipase Positional Selectivity for 1,3-Diolein Synthesis. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1125270
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125270