The Use of a Hypoallergenic Dermal Matrix for Wrapping in Peripheral Nerve Lesions Regeneration: Functional and Quantitative Morphological Analysis in an Experimental Animal Model
Joint Authors
Lo Giudice, Roberto
Colonna, Michele R.
Fazio, Antonina
Costa, Alfio Luca
Galletti, Franco
Galletti, Bruno
Galletti, Cosimo
Lo Giudice, Giorgio
Dell’Aversana Orabona, Giovanni
Papalia, Igor
Geuna, Stefano
Ronchi, Giulia
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Introduction.
The aim of this research was to test, in an animal model, the nerve regeneration technique with a hypoallergenic acellular dermal matrix used to wrap the microsurgical neural suture.
Materials and Methods.
Two groups of rats received the cut of limb right median nerves.
The regeneration technique considers for both groups an end-to-end nerve suture.
In the experimental group (A) was used also a wrapping protocol by a conduit of collagen matrix currently used in oral surgery.
The animals underwent functional grasping tests (at 1, 3, 5, and 7 months) and a histological and quantitative analysis of distal nerve was performed at the end of experimental time.
Result.
After seven months, the grasping test reveals functional recovery in each tested animal; this improvement is more evident in Group A.
The fibers appear well organized with restored myelin sheaths in both groups.
Group A showed a great quantity of connective tissue surrounding the nerve.
The quantitative morphology analysis in both groups shows a similar fibers density, fiber diameter, and myelin thickness.
The differences between the groups in axon mean diameter are significant.
In Group A M/d, D/d, and g-ratio is significantly higher compared to control group.
Conclusions.
Histological and functional assessments show a functional recovery of the injured nerve in the test groups, stressed by the results of the grasping tests and the meaningful increasing in fiber diameter and higher g-ratio.
Moreover, a connective tissue cuff distinguishes the distal portion of the injured nerve.
Considering the easy availability and handling of the material used in this study we can conclude that this experimental technique can be considered as a valid alternative to protect nerves in nerve wrap surgery.
American Psychological Association (APA)
Colonna, Michele R.& Fazio, Antonina& Costa, Alfio Luca& Galletti, Franco& Lo Giudice, Roberto& Galletti, Bruno…[et al.]. 2019. The Use of a Hypoallergenic Dermal Matrix for Wrapping in Peripheral Nerve Lesions Regeneration: Functional and Quantitative Morphological Analysis in an Experimental Animal Model. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125571
Modern Language Association (MLA)
Colonna, Michele R.…[et al.]. The Use of a Hypoallergenic Dermal Matrix for Wrapping in Peripheral Nerve Lesions Regeneration: Functional and Quantitative Morphological Analysis in an Experimental Animal Model. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1125571
American Medical Association (AMA)
Colonna, Michele R.& Fazio, Antonina& Costa, Alfio Luca& Galletti, Franco& Lo Giudice, Roberto& Galletti, Bruno…[et al.]. The Use of a Hypoallergenic Dermal Matrix for Wrapping in Peripheral Nerve Lesions Regeneration: Functional and Quantitative Morphological Analysis in an Experimental Animal Model. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125571
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125571