Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3KAkt and MAPKErk Signaling Pathways
Joint Authors
Sun, Gui-Bo
Yu, Wenzhen
Bai, Yu-Jing
Li, Yang
Jiang, Yan-rong
Shi, Xuan
Chen, Li
Feng, Jing
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-04
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Diabetic macular edema is major cause of vision loss associated with diabetic retinopathy.
Breakdown of blood-retinal barrier, especially inner BRB, is an early event in pathogenesis of DR.
Apelin, an endogenous ligand of APJ, mediates angiogenesis and is involved in the development of DR.
The present study aimed to investigate effects and mechanism of apelin-13 in vascular permeability during DME.
We verified apelin-13 was upregulated in DME patients’ vitreous.
High glucose incubation led to a progressive increase of apelin-13, APJ, cytoskeleton, and tight junction proteins, including VE-Cadherin, FAK, Src, ZO-1, and occludin.
Apelin-13 promoted HRMEC proliferation and migration and phosphorylation of both cytoskeleton and tight junction under both normal and high glucose conditions.
Besides, apelin-13 activated PI-3K/Akt and MAPK/Erk signaling pathways, including PLCγ1, p38, Akt, and Erk both in HRMEC and in C57BL/6 mice.
Meanwhile, F13A performed opposite effects compared with apelin-13.
In in vivo study, apelin-13 was also upregulated in retina of db/db mice.
Taken together, apelin-13 increased biologic activity of HRMEC, as well as expression of both cytoskeleton and tight junction in DME via PI-3K/Akt and MAPK/Erk signaling pathways.
Apelin-13 as an early promoter of vascular permeability may offer a new perspective strategy in early treatment of DR.
American Psychological Association (APA)
Li, Yang& Bai, Yu-Jing& Jiang, Yan-rong& Yu, Wenzhen& Shi, Xuan& Chen, Li…[et al.]. 2018. Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3KAkt and MAPKErk Signaling Pathways. BioMed Research International،Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1125723
Modern Language Association (MLA)
Li, Yang…[et al.]. Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3KAkt and MAPKErk Signaling Pathways. BioMed Research International No. 2018 (2018), pp.1-17.
https://search.emarefa.net/detail/BIM-1125723
American Medical Association (AMA)
Li, Yang& Bai, Yu-Jing& Jiang, Yan-rong& Yu, Wenzhen& Shi, Xuan& Chen, Li…[et al.]. Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3KAkt and MAPKErk Signaling Pathways. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1125723
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125723