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Effect of eNOS on Ischemic Postconditioning-Induced Autophagy against IschemiaReperfusion Injury in Mice
Joint Authors
Shao, Jun
Miao, Chen
Geng, Zhi
Gu, Maohong
Wu, Yanhu
Li, Qingguo
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Autophagy is involved in the development of numerous illnesses, including ischemia/reperfusion (I/R).
Endothelial nitric oxide synthase (eNOS) participates in the protective effects of ischemic postconditioning (IPostC).
However, it remains unclear whether eNOS-mediated autophagy serves as a critical role in IPostC in the hearts of mice, in protecting against I/R injury.
In the present study, the hearts of mice with left anterior descending coronary artery ligation were studied as I/R models.
H9c2 cells underwent exposure to hypoxia/reoxygenation (H/R) and were examined as in vitro model.
IPostC reduced mice myocardial infarct size and improved the structure of the heart.
IPostC increased the formation of autophagosomes and increased the phosphorylation of eNOS and adenosine monophosphate-activated protein kinase (AMPK).
Autophagy and eNOS inhibition suppressed the cardioprotective effects of IPostC.
AMPK or eNOS inhibition abolished the improvement effect of IPostC on autophagy.
AMPK inhibition decreased eNOS phosphorylation in the heart.
Additionally, H9c2 cells suffering hypoxia were used as in vitro model.
Autophagy or eNOS inhibition abolished the protective effects of hypoxic postconditioning (HPostC) against H/R injury.
AMPK and eNOS inhibition/knockout decreased autophagic activity in the HPostC group.
These results indicated that IPostC protects the heart against I/R injury, partially via promoting AMPK/eNOS-mediated autophagy.
American Psychological Association (APA)
Shao, Jun& Miao, Chen& Geng, Zhi& Gu, Maohong& Wu, Yanhu& Li, Qingguo. 2019. Effect of eNOS on Ischemic Postconditioning-Induced Autophagy against IschemiaReperfusion Injury in Mice. BioMed Research International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1125888
Modern Language Association (MLA)
Shao, Jun…[et al.]. Effect of eNOS on Ischemic Postconditioning-Induced Autophagy against IschemiaReperfusion Injury in Mice. BioMed Research International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1125888
American Medical Association (AMA)
Shao, Jun& Miao, Chen& Geng, Zhi& Gu, Maohong& Wu, Yanhu& Li, Qingguo. Effect of eNOS on Ischemic Postconditioning-Induced Autophagy against IschemiaReperfusion Injury in Mice. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1125888
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125888