![](/images/graphics-bg.png)
Inhibitory Effects of Compounds and Extracts from Ampelopsis brevipedunculata on IL-6-Induced STAT3 Activation
Joint Authors
Jung, Kyungsook
Jang, Hyun-Jae
Lee, Seung-Jae
Lim, Hyung-Jin
Lee, Soyoung
Park, Chan Sun
Lee, Seung Woong
Rho, Mun-Chual
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Ampelopsis brevipedunculata (Maxim.) Trautv.
(AB), a traditional East Asian medicine, exhibits protective effects against several inflammatory diseases.
Our search for an inhibitor of IL-6-induced JAK2/STAT3 activation revealed that AB ethanolic extract (ABE) had a significant inhibitory effect on IL-6-induced STAT3 expression in Hep3B cells.
The isolation and purification of an EtOAc-soluble fraction of ABE (ABEA) using reversed-phase high-performance liquid chromatography (RP-HPLC) afforded 17 compounds.
The structures of these compounds (1-17) were elucidated based on 1H and 13C nuclear magnetic resonance (NMR) spectroscopy as well as electrospray-ionization mass spectrometry (ESI-MS) data.
ABE and ABEA were screened by a luciferase assay using Hep3B cells transfected with the STAT3 reporter gene.
ABEA exhibited potent inhibitory effects on IL-6-induced STAT3 expression; moreover, these effects arose from the inhibition of the phosphorylation of the STAT3, JAK2, and ERK proteins in U266 cells.
In addition, the compounds isolated from ABEA were measured for their inhibitory effects on IL-6-stimulated STAT3 expression.
Of the compounds isolated, betulin showed the greatest inhibitory effects on IL-6-induced STAT3 activation in the luciferase assay (IC50 value: 3.12 μM).
Because of its potential for inhibiting STAT3 activation, A.
brevipedunculata could be considered a source of compounds of pharmaceutical interest.
American Psychological Association (APA)
Jang, Hyun-Jae& Lee, Seung-Jae& Lim, Hyung-Jin& Jung, Kyungsook& Lee, Soyoung& Park, Chan Sun…[et al.]. 2018. Inhibitory Effects of Compounds and Extracts from Ampelopsis brevipedunculata on IL-6-Induced STAT3 Activation. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1126005
Modern Language Association (MLA)
Jang, Hyun-Jae…[et al.]. Inhibitory Effects of Compounds and Extracts from Ampelopsis brevipedunculata on IL-6-Induced STAT3 Activation. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1126005
American Medical Association (AMA)
Jang, Hyun-Jae& Lee, Seung-Jae& Lim, Hyung-Jin& Jung, Kyungsook& Lee, Soyoung& Park, Chan Sun…[et al.]. Inhibitory Effects of Compounds and Extracts from Ampelopsis brevipedunculata on IL-6-Induced STAT3 Activation. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1126005
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126005