Effects of Juniperus phoenicea Hydroalcoholic Extract on Inflammatory Mediators and Oxidative Stress Markers in Carrageenan-Induced Paw Oedema in Mice

Abstract EN

Juniperus phoenicea (J.

phoenicea) is a wild tree belonging to the Cupressaceae family, commonly used for the treatment of several disorders.

This study aimed to evaluate the potential protective effects of J.

phoenicea hydroethanolic extract (EtOH-H2OE) against oxidation, acute inflammation, and pain in mice models.

For the purpose, chemical compounds of J.

phoenicea EtOH-H2OE were also analyzed by GC-MS.

The J.

phoenicea EtOH-H2OE showed a potent antioxidant activity in vitro, thanks to its richness in phenolic and flavonoid compounds.

Mice treated with EtOH-H2OE (100 mg/kg BW) showed reduced paw oedema formation and decreased malondialdehyde (MDA) content.

The evaluation of antioxidant enzyme activities in paw oedema tissue after five hours of carrageenan induction showed a significant increase (P<0.05).

Inflammatory biomarkers explorations of J.

phoenicea EtOH-H2OE-treated mice showed a restoration of the studied parameters to near-normal values.

Furthermore, EtOH-H2OE of J.

phoenicea produced a significant reduction of the number of abdominal writhes (P<0.05) in a dose-dependent way.

Phytochemical analysis of the J.

phoenicea EtOH-H2OE by GC-MS showed the presence of hexadecanoic and stearic acids known as anti-inflammatory and analgesic compounds.

Our investigation provided evidence that J.

phoenicea EtOH-H2OE can effectively reduce the inflammation and pain in mice models.