A Human Umbilical Cord Mesenchymal Stem Cell-Conditioned MediumChitosanCollagenβ-Glycerophosphate Thermosensitive Hydrogel Promotes Burn Injury Healing in Mice
Joint Authors
Zhou, Panpan
Li, Xue
Zhang, Bing
Shi, Qing
Li, Dong
Ju, Xiuli
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-02
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
We investigated the effects of a human umbilical cord mesenchymal stem cell-conditioned medium (MSC-CM)/chitosan/collagen/β-glycerophosphate (β-GP) thermosensitive hydrogel (MSC-CM/hydrogel) on mice with third-degree burns.
MSC-CM was collected and mixed with chitosan, collagen, and β-GP to generate the thermosensitive MSC-CM/hydrogel, which was stored in the liquid phase at 4°C.
The wounds of established third-degree burned mice were then externally covered with the MSC-CM/hydrogel, which formed a gel when placed on the wounds at physiological temperature.
Injured mice in three additional groups were treated with unconditioned MSC medium (UM), MSC-CM, or UM/chitosan/collagen/β-GP thermosensitive hydrogels.
Skin wound samples were obtained 4, 14, and 28 days after burning for further analysis by hematoxylin and eosin and Ki-67 staining.
Wound healing rates and times, in addition to immunohistochemical results, were then compared and analyzed among the four groups.
Application of the MSC-CM/hydrogel shortened healing time, limited the area of inflammation, enhanced reepithelialization, promoted the formation of high-quality, well-vascularized granulation tissue, and attenuated the formation of fibrotic and hypertrophic scar tissue.
In summary, MSC-CM/hydrogel effectively promotes wound healing in third-degree burned mice.
American Psychological Association (APA)
Zhou, Panpan& Li, Xue& Zhang, Bing& Shi, Qing& Li, Dong& Ju, Xiuli. 2019. A Human Umbilical Cord Mesenchymal Stem Cell-Conditioned MediumChitosanCollagenβ-Glycerophosphate Thermosensitive Hydrogel Promotes Burn Injury Healing in Mice. BioMed Research International،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1126246
Modern Language Association (MLA)
Zhou, Panpan…[et al.]. A Human Umbilical Cord Mesenchymal Stem Cell-Conditioned MediumChitosanCollagenβ-Glycerophosphate Thermosensitive Hydrogel Promotes Burn Injury Healing in Mice. BioMed Research International No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1126246
American Medical Association (AMA)
Zhou, Panpan& Li, Xue& Zhang, Bing& Shi, Qing& Li, Dong& Ju, Xiuli. A Human Umbilical Cord Mesenchymal Stem Cell-Conditioned MediumChitosanCollagenβ-Glycerophosphate Thermosensitive Hydrogel Promotes Burn Injury Healing in Mice. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1126246
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126246