Development of a Novel Diagnostic Biomarker Set for Rheumatoid Arthritis Using a Proteomics Approach
Joint Authors
Kang, Hee Kyu
Mun, Sora
Lee, Jiyeong
Lim, Mi-Kyoung
Lee, You-Rim
Ihm, Chunhwa
Lee, Seung Hoon
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Rheumatoid arthritis (RA) is an autoimmune disease that starts with inflammation of the synovial membrane.
Studies have been conducted to develop methods for efficient diagnosis of RA and to identify the mechanisms underlying RA development.
Blood samples can be useful for detecting disturbance of homeostasis in patients with RA.
Nanoliquid chromatography-tandem mass spectrometry (LC-MS/MS) is an efficient proteomics approach to analyze blood sample and quantify serum proteins.
Methods.
Serum samples of 18 healthy controls and 18 patients with RA were analyzed by LC-MS/MS.
Selected candidate biomarkers were validated by enzyme-linked immunosorbent assay (ELISA) using sera from 43 healthy controls and 44 patients with RA.
Results.
Thirty-eight proteins were significantly differentially expressed by more than 2-fold in healthy controls and patients with RA.
Based on a literature survey, we selected six candidate RA biomarkers.
ELISA was used to evaluate whether these proteins effectively allow distinguishing patients with RA from healthy controls and monitoring drug efficacy.
SAA4, gelsolin, and vitamin D-binding protein were validated as potential biomarkers of RA for screening and drug efficacy monitoring of RA.
Conclusions.
We identified a panel of three biomarkers for RA which has potential for application in RA diagnosis and drug efficacy monitoring.
Further, our findings will aid in understanding the pathogenesis of RA.
American Psychological Association (APA)
Mun, Sora& Lee, Jiyeong& Lim, Mi-Kyoung& Lee, You-Rim& Ihm, Chunhwa& Lee, Seung Hoon…[et al.]. 2018. Development of a Novel Diagnostic Biomarker Set for Rheumatoid Arthritis Using a Proteomics Approach. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1128557
Modern Language Association (MLA)
Mun, Sora…[et al.]. Development of a Novel Diagnostic Biomarker Set for Rheumatoid Arthritis Using a Proteomics Approach. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1128557
American Medical Association (AMA)
Mun, Sora& Lee, Jiyeong& Lim, Mi-Kyoung& Lee, You-Rim& Ihm, Chunhwa& Lee, Seung Hoon…[et al.]. Development of a Novel Diagnostic Biomarker Set for Rheumatoid Arthritis Using a Proteomics Approach. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1128557
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128557