Positive Effects against UV-A Induced Damage and Oxidative Stress on an In Vitro Cell Model Using a Hyaluronic Acid Based Formulation Containing Amino Acids, Vitamins, and Minerals
Joint Authors
Stellavato, Antonietta
Vassallo, Valentina
De Rosa, Mario
Schiraldi, Chiara
Pirozzi, Anna Virginia Adriana
Donato, Stella
Scognamiglio, Ilaria
Reale, Sabrina
Di Pardo, Alba
Filosa, Stefania
Bellia, Gilberto
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Ultraviolet (UV) radiations are responsible for skin photoaging inducing alteration of the molecular and cellular pathways resulting in dryness and reduction of skin elasticity.
In this study, we investigated, in vitro, the antiaging and antioxidant effects of hyaluronan formulations based hydrogel.
Skinkò E, an intradermic formulation composed of hyaluronic acid (HA), minerals, amino acids, and vitamins, was compared with the sole HA of the same size.
For this purpose, HaCaT cells were subjected to UV-A radiations and H2O2 exposure and then treated with growth medium (CTR) combined with M-HA or Skinkò E to evaluate their protective ability against stressful conditions.
Cells reparation was evaluated using a scratch in vitro model and Time-Lapse Video Microscopy.
A significant protective effect for Skinkò E was shown with respect to M-HA.
In addition, Skinkò E increased cell reparation.
Therefore, NF-kB, SOD-2, and HO-1 were significantly reduced at the transcriptional and protein level.
Interestingly, γ-H2AX and protein damage assay confirmed the protection by hyaluronans tested against oxidative stress.
G6pdΔ ES cell line, highly susceptible to oxidative stress, was used as a further cellular model to assess the antioxidant effect of Skinkò E.
Western blotting analyses showed that the treatment with this new formulation exerts marked antioxidant action in cells exposed to UV-A and H2O2.
Thus, the protective and reparative properties of Skinkò E make it an interesting tool to treat skin aging.
American Psychological Association (APA)
Stellavato, Antonietta& Pirozzi, Anna Virginia Adriana& Donato, Stella& Scognamiglio, Ilaria& Reale, Sabrina& Di Pardo, Alba…[et al.]. 2018. Positive Effects against UV-A Induced Damage and Oxidative Stress on an In Vitro Cell Model Using a Hyaluronic Acid Based Formulation Containing Amino Acids, Vitamins, and Minerals. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1129182
Modern Language Association (MLA)
Stellavato, Antonietta…[et al.]. Positive Effects against UV-A Induced Damage and Oxidative Stress on an In Vitro Cell Model Using a Hyaluronic Acid Based Formulation Containing Amino Acids, Vitamins, and Minerals. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1129182
American Medical Association (AMA)
Stellavato, Antonietta& Pirozzi, Anna Virginia Adriana& Donato, Stella& Scognamiglio, Ilaria& Reale, Sabrina& Di Pardo, Alba…[et al.]. Positive Effects against UV-A Induced Damage and Oxidative Stress on an In Vitro Cell Model Using a Hyaluronic Acid Based Formulation Containing Amino Acids, Vitamins, and Minerals. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1129182
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129182