Stromal Cell-Derived Factor-1α Alleviates Calcium-Sensing Receptor Activation-Mediated IschemiaReperfusion Injury by Inhibiting Caspase-3Caspase-9-Induced Cell Apoptosis in Rat Free Flaps
Joint Authors
Song, Li
Gao, Li-Na
Wang, Jun
Thapa, Swosti
Li, Yong
Zhong, Xiao-Bo
Zhao, Hong-Wei
Xiang, Xue-Rong
Zhang, Fu-Gui
Ji, Ping
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Surgical flaps are frequently affected by ischemia/reperfusion (I/R) injury.
Calcium-sensing receptor (CaSR) and stromal cell-derived factor-1α (SDF-1α) are closely associated with myocardial I/R injury.
This study was performed to evaluate the feasibility of applying SDF-1α to counteract CaSR activation-mediated I/R injury in ischemic free flaps.
Free flaps that underwent ischemia for 3 h were equally randomized into five groups: CaCl2, NPS2143 + CaCl2, SDF-1α + CaCl2, AMD3100 + SDF-1α + CaCl2, and normal saline.
The free flaps were harvested to evaluate flap necrosis and neovascularization after 2 h or 7 d of reperfusion.
p-CaSR/CaSR was extensively expressed in vascular endothelial cells of free flaps after I/R injury, and activation of the SDF-1α/CXCR4 axis and NPS2143 could reduce the expression of cleaved caspase-3, caspase-9, FAS, Cyt-c, and Bax and increase Bcl-2 expression; the opposite was true after CaSR activation.
Interestingly, initiation of the SDF-1α/CXCR4 axis might abrogate CaSR activation-induced I/R injury through enhancement of microvessel density.
In conclusion, CaSR might become a novel therapeutic target of free flaps affected by I/R injury.
Activation of the SDF-1α/CXCR4 axis and NPS2143 could counteract CaSR activation-mediated I/R injury and promote free flap survival through inhibition of caspase-3/caspase-9-related cell apoptosis and enhancement of neovascularization.
American Psychological Association (APA)
Song, Li& Gao, Li-Na& Wang, Jun& Thapa, Swosti& Li, Yong& Zhong, Xiao-Bo…[et al.]. 2018. Stromal Cell-Derived Factor-1α Alleviates Calcium-Sensing Receptor Activation-Mediated IschemiaReperfusion Injury by Inhibiting Caspase-3Caspase-9-Induced Cell Apoptosis in Rat Free Flaps. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1129401
Modern Language Association (MLA)
Song, Li…[et al.]. Stromal Cell-Derived Factor-1α Alleviates Calcium-Sensing Receptor Activation-Mediated IschemiaReperfusion Injury by Inhibiting Caspase-3Caspase-9-Induced Cell Apoptosis in Rat Free Flaps. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1129401
American Medical Association (AMA)
Song, Li& Gao, Li-Na& Wang, Jun& Thapa, Swosti& Li, Yong& Zhong, Xiao-Bo…[et al.]. Stromal Cell-Derived Factor-1α Alleviates Calcium-Sensing Receptor Activation-Mediated IschemiaReperfusion Injury by Inhibiting Caspase-3Caspase-9-Induced Cell Apoptosis in Rat Free Flaps. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1129401
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129401