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A Novel Fragment Derived from Laminin-411 Facilitates Proliferation and Differentiation of Odontoblast-Like Cells
Joint Authors
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The aim for the present study was to evaluate the in vitro effects of iMatrix-411 in odontoblast-like cells.
To that end, iMatrix-411 was coated to both nontissue culture treated- (Non-PS) and tissue culture treated-polystyrene (TCPS) multiwells.
MDPC-23 cells were seeded into noncoated (control) or coated wells.
Optimal coating density and cell proliferation were assessed by cell counting kit-8 (CCK-8) at day two, day three, and day five.
Osteo/odontogenic differentiation was evaluated by real-time RT-PCR and alkaline phosphatase (ALP) activity at days seven and eight, respectively.
Calcific deposition of cells was visualized by alizarin red staining.
Data were analyzed with post hoc Tukey HSD test (p<0.05).
Optimal coating density for iMatrix-411 was 8 μg/cm2.
Exposure of MDPC-23 cells to iMatrix-411 in either non-PS or TCPS significantly enhanced proliferative activity.
iMatrix-411 elevated ALP activity in both types of culture plates.
iMatrix-411 significantly increased the mRNA level of OCN, BSP, OPN, ALP, and DMP-1.
Meanwhile, it enhanced the expression of several integrin subunits: ITGA1, ITGA5, ITGAV, ITGB1, and ITGB5.
Finally, iMatrix-411 also accelerated the mineralization at day eight in Non-PS.
The results indicated iMatrix-411 stimulates proliferation and favours differentiation of odontoblast-like cells.
American Psychological Association (APA)
Tang, Jia& Saito, Takashi. 2018. A Novel Fragment Derived from Laminin-411 Facilitates Proliferation and Differentiation of Odontoblast-Like Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129773
Modern Language Association (MLA)
Tang, Jia& Saito, Takashi. A Novel Fragment Derived from Laminin-411 Facilitates Proliferation and Differentiation of Odontoblast-Like Cells. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1129773
American Medical Association (AMA)
Tang, Jia& Saito, Takashi. A Novel Fragment Derived from Laminin-411 Facilitates Proliferation and Differentiation of Odontoblast-Like Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1129773
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1129773