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Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast Europe
Joint Authors
Boldogkői, Zsolt
Csabai, Zsolt
Tombácz, Dóra
Deim, Zoltán
Snyder, Michael
Source
Canadian Journal of Infectious Diseases and Medical Microbiology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Pseudorabies virus (PRV) is the causative agent of Aujeszky’s disease giving rise to significant economic losses worldwide.
Many countries have implemented national programs for the eradication of this virus.
In this study, long-read sequencing was used to determine the nucleotide sequence of the genome of a novel PRV strain (PRV-MdBio) isolated in Serbia.
Results.
In this study, a novel PRV strain was isolated and characterized.
PRV-MdBio was found to exhibit similar growth properties to those of another wild-type PRV, the strain Kaplan.
Single-molecule real-time (SMRT) sequencing has revealed that the new strain differs significantly in base composition even from strain Kaplan, to which it otherwise exhibits the highest similarity.
We compared the genetic composition of PRV-MdBio to strain Kaplan and the China reference strain Ea and obtained that radical base replacements were the most common point mutations preceding conservative and silent mutations.
We also found that the adaptation of PRV to cell culture does not lead to any tendentious genetic alteration in the viral genome.
Conclusion.
PRV-MdBio is a wild-type virus, which differs in base composition from other PRV strains to a relatively large extent.
American Psychological Association (APA)
Csabai, Zsolt& Tombácz, Dóra& Deim, Zoltán& Snyder, Michael& Boldogkői, Zsolt. 2019. Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast Europe. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1130010
Modern Language Association (MLA)
Csabai, Zsolt…[et al.]. Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast Europe. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1130010
American Medical Association (AMA)
Csabai, Zsolt& Tombácz, Dóra& Deim, Zoltán& Snyder, Michael& Boldogkői, Zsolt. Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast Europe. Canadian Journal of Infectious Diseases and Medical Microbiology. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1130010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130010