Salivary MicroRNAs for Early Detection of Head and Neck Squamous Cell Carcinoma: A Case-Control Study in the High Altitude Mestizo Ecuadorian Population
Joint Authors
García-Cárdenas, Jennyfer M.
Guevara-Ramírez, Patricia
López-Cortés, Andrés
Leone, Paola E.
Paz-y-Miño, César
Salazar-Ruales, Carolina
Arguello, Jessica-Viviana
Cabrera-Andrade, Alejandro
Peralta, Patricio
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer with the highest incidence worldwide.
HNSCC is often diagnosed at advanced stages, incurring significant high mortality and morbidity.
The use of saliva, as a noninvasive tool for the diagnosis of cancer, has recently increased.
Salivary microRNAs (miRNAs) have emerged as a promising molecular tool for early diagnosis of HNSCC.
The aim was to identify the differential expression of salivary miRNAs associated with HNSCC in the high altitude mestizo Ecuadorian population.
Using PCR Arrays, miR-122-5p, miR-92a-3p, miR-124-3p, miR-205-5p, and miR-146a-5p were found as the most representative ones.
Subsequently, miRNAs expression was confirmed in saliva samples from 108 cases and 108 controls.
miR-122-5p, miR-92a-3p, miR-124-3p, and miR-146a-5p showed significant statistical difference between cases and controls with areas under the curve (AUC) of 0.73 (p < 0.001), 0.70 (p < 0.001), 0.71 (p = 0.002), and 0.66 (p = 0.008), respectively.
miRNAs were also deregulated in between HNSCC localizations.
A differentiated expression of miR-122-5p between oral cancer and oropharynx cancer (AUC of 0.96 p = 0.01) was found: miR-124-3p between larynx and pharynx (AUC = 0.97, p < 0.01) and miR-146a-5p between larynx, oropharynx, and oral cavity (AUC = 0.96, p = 0.01).
Moreover, miR-122-5p, miR-124-3p, miR-205-5p, and miR-146a-5p could differentiate between HPV+ and HPV- (p=0.004).
Finally, the expression profiles of the five miRNAs were evaluated to discriminate HNSCC patient’s tumor stages (TNM 2-4).
miR-122-5p differentiates TNM 2 and 3 (p = 0.002, AUC = 0.92), miR-124-3p TNM 2, 3, and 4 (p < 0.001, AUC = 98), miR-146a-5p TNM 2 and 3 (p < 0.001, AUC = 0.97), and miR-92a-3p TNM 3 (p < 0.001, AUC = 0.99).
Taken together, these findings show that altered expression of miRNAs could be used as biomarkers for HNSCC diagnosis in the high altitude mestizo Ecuadorian population.
American Psychological Association (APA)
Salazar-Ruales, Carolina& Arguello, Jessica-Viviana& López-Cortés, Andrés& Cabrera-Andrade, Alejandro& García-Cárdenas, Jennyfer M.& Guevara-Ramírez, Patricia…[et al.]. 2018. Salivary MicroRNAs for Early Detection of Head and Neck Squamous Cell Carcinoma: A Case-Control Study in the High Altitude Mestizo Ecuadorian Population. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1130024
Modern Language Association (MLA)
Salazar-Ruales, Carolina…[et al.]. Salivary MicroRNAs for Early Detection of Head and Neck Squamous Cell Carcinoma: A Case-Control Study in the High Altitude Mestizo Ecuadorian Population. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1130024
American Medical Association (AMA)
Salazar-Ruales, Carolina& Arguello, Jessica-Viviana& López-Cortés, Andrés& Cabrera-Andrade, Alejandro& García-Cárdenas, Jennyfer M.& Guevara-Ramírez, Patricia…[et al.]. Salivary MicroRNAs for Early Detection of Head and Neck Squamous Cell Carcinoma: A Case-Control Study in the High Altitude Mestizo Ecuadorian Population. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1130024
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130024