Assessment of Intratumoral Doxorubicin Penetration after Mild Hyperthermia-Mediated Release from Thermosensitive Liposomes
Joint Authors
Moonen, Chrit T. W.
Derieppe, Marc
Escoffre, Jean-Michel
Denis de Senneville, Baudouin
van Houtum, Quincy
Rijbroek, Angelique Barten-van
van der Wurff-Jacobs, Kim
Dubois, Ludwig
Bos, Clemens
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-07
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In solid tumors, rapid local intravascular release of anticancer agents, e.g., doxorubicin (DOX), from thermosensitive liposomes (TSLs) can be an option to overcome poor extravasation of drug nanocarriers.
The driving force of DOX penetration is the drug concentration gradient between the vascular compartment and the tumor interstitium.
In this feasibility study, we used fibered confocal fluorescence microscopy (FCFM) to monitor in real-time DOX penetration in the interstitium of a subcutaneous tumor after its intravascular release from TSLs, Thermodox®.
Cell uptake kinetics of the released DOX was quantified, along with an in-depth assessment of released-DOX penetration using an evolution model.
A subcutaneous rat R1 rhabdomyosarcoma xenograft was used.
The rodent was positioned in a setup including a water bath, and FCFM identification of functional vessels in the tumor tissue was applied based on AngioSense.
The tumor-bearing leg was immersed in the 43°C water for preheating, and TSLs were injected intravenously.
Real-time monitoring of intratumoral (i.t.) DOX penetration could be performed, and it showed the progressing DOX wave front via its native fluorescence, labeling successively all cell nuclei.
Cell uptake rates (1/k) of 3 minutes were found (n=241 cells), and a released-DOX penetration in the range of 2500 µm2·s−1 was found in the tumor extravascular space.
This study also showed that not all vessels, identified as functional based on AngioSense, gave rise to local DOX penetration.
American Psychological Association (APA)
Derieppe, Marc& Escoffre, Jean-Michel& Denis de Senneville, Baudouin& van Houtum, Quincy& Rijbroek, Angelique Barten-van& van der Wurff-Jacobs, Kim…[et al.]. 2019. Assessment of Intratumoral Doxorubicin Penetration after Mild Hyperthermia-Mediated Release from Thermosensitive Liposomes. Contrast Media & Molecular Imaging،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1130187
Modern Language Association (MLA)
Derieppe, Marc…[et al.]. Assessment of Intratumoral Doxorubicin Penetration after Mild Hyperthermia-Mediated Release from Thermosensitive Liposomes. Contrast Media & Molecular Imaging No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1130187
American Medical Association (AMA)
Derieppe, Marc& Escoffre, Jean-Michel& Denis de Senneville, Baudouin& van Houtum, Quincy& Rijbroek, Angelique Barten-van& van der Wurff-Jacobs, Kim…[et al.]. Assessment of Intratumoral Doxorubicin Penetration after Mild Hyperthermia-Mediated Release from Thermosensitive Liposomes. Contrast Media & Molecular Imaging. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1130187
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1130187