Increased CCR 7 lo PD- 1 hi CXCR 5 + CD 4 + T Cells in Peripheral Blood Mononuclear Cells Are Correlated with Immune Activation in Patients with Chronic HBV Infection

Abstract EN

T follicular helper cells (Tfh cells) affect essential immune pathogenesis in chronic hepatitis B virus (HBV) infection.

The CCR 7 l o PD- 1 h i Tfh subset has a partial Tfh effector phenotype and is associated with active Tfh differentiation, whereas the CCR 7 h i PD- 1 l o Tfh subset is a resting phenotype.

We recruited 20 healthy volunteers and 77 patients with chronic HBV infection, including those in the immune tolerant (IT) phase (n=19), immune clearance (IC) phase (n=20), low replicative (LR) phase (n=18), and reactivation (RA) phase (n=20).

The expression of CD4, CXCR5, PD-1, and CCR7 was detected in T cells from peripheral blood by flow cytometry.

The frequency of the CCR 7 l o PD- 1 h i T subset was significantly higher in the patients than in the healthy controls (14.92±4.87% vs 12.23±2.95%, p=0.018).

The frequency of this Tfh subset in the IC group (18.42%±3.08) was increased compared with the IT group (11.94±2.87%, p=0.001) and LR group (13.65±4.93%, p=0.031) and was higher in the RA group than in the IT group (16.03±5.37% vs 11.94±2.87%, p=0.030).

We observed a weak positive correlation between the CCR 7 l o PD- 1 h i Tfh subset population and the alanine transaminase (ALT) level (r=0.370, p=0.001).

The CCR 7 l o PD- 1 h Tfh subset in the chronic HBV-infected patients was elevated to various degrees among the different immune phases.

CCR 7 l o PD- 1 h i CXCR5+CD4+ T cells are correlated with the immune status of chronic HBV infection patients and may be developed as a potential indicator for antiviral treatment.