In Vivo 6-([18F]Fluoroacetamido)-1-hexanoicanilide PET Imaging of Altered Histone Deacetylase Activity in Chemotherapy-Induced Neurotoxicity
Joint Authors
Fukumitsu, Nobuyoshi
Jeong, Hwan-Jeong
Gelovani, Juri G.
Flores II, Leo Garcia
Mukhopadhyay, Uday
Young, Daniel
Tong, William
Yeh, Skye Hsin-Hsien
Ogawa, Kazuma
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Histone deacetylases (HDACs) regulate gene expression by changing histone deacetylation status.
Neurotoxicity is one of the major side effects of cisplatin, which reacts with deoxyribonucleic acid (DNA) and has excellent antitumor effects.
Suberoylanilide hydroxamic acid (SAHA) is an HDAC inhibitor with neuroprotective effects against cisplatin-induced neurotoxicity.
Purpose.
We investigated how cisplatin with and without SAHA pretreatment affects HDAC expression/activity in the brain by using 6-([18F]fluoroacetamido)-1-hexanoicanilide ([18F]FAHA) as a positron emission tomography (PET) imaging agent for HDAC IIa.
Materials and Methods.
[18F]FAHA and [18F]fluoro-2-deoxy-2-D-glucose ([18F]FDG) PET studies were done in 24 mice on 2 consecutive days and again 1 week later.
The mice were divided into three groups according to drug administration between the first and second imaging sessions (Group A: cisplatin 2 mg/kg, twice; Group B: cisplatin 4 mg/kg, twice; Group C: cisplatin 4 mg/kg, twice, and SAHA 300 mg/kg pretreatment, 4 times).
Results.
The K i value of [18F]FAHA was increased and the percentage of injected dose/tissue g (% ID/g) of [18F]FDG was decreased in the brains of animals in Groups A and B.
The K i value of [18F]FAHA and % ID/g of [18F]FDG were not significantly different in Group C.
Conclusions.
[18F]FAHA PET clearly showed increased HDAC activity suggestive of cisplatin neurotoxicity in vivo, which was blocked by SAHA pretreatment.
American Psychological Association (APA)
Fukumitsu, Nobuyoshi& Yeh, Skye Hsin-Hsien& Flores II, Leo Garcia& Mukhopadhyay, Uday& Young, Daniel& Ogawa, Kazuma…[et al.]. 2018. In Vivo 6-([18F]Fluoroacetamido)-1-hexanoicanilide PET Imaging of Altered Histone Deacetylase Activity in Chemotherapy-Induced Neurotoxicity. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1131366
Modern Language Association (MLA)
Fukumitsu, Nobuyoshi…[et al.]. In Vivo 6-([18F]Fluoroacetamido)-1-hexanoicanilide PET Imaging of Altered Histone Deacetylase Activity in Chemotherapy-Induced Neurotoxicity. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1131366
American Medical Association (AMA)
Fukumitsu, Nobuyoshi& Yeh, Skye Hsin-Hsien& Flores II, Leo Garcia& Mukhopadhyay, Uday& Young, Daniel& Ogawa, Kazuma…[et al.]. In Vivo 6-([18F]Fluoroacetamido)-1-hexanoicanilide PET Imaging of Altered Histone Deacetylase Activity in Chemotherapy-Induced Neurotoxicity. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1131366
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131366