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Evaluation of 99mTc-HYNIC-VCAM-1scFv as a Potential Qualitative and Semiquantitative Probe Targeting Various Tumors
Joint Authors
Zhang, Yongxue
Lan, Xiaoli
Zhang, Xiao
Hu, Fan
Liu, Chunbao
Yin, Lianglan
Zhang, Yingying
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Vascular cell adhesion molecule 1 (VCAM-1) is overexpressed in varieties of cancers.
This study aimed to evaluate the application of a single chain variable fragment (scFv) of anti-VCAM-1 antibody labeled with 99mTc as a possible imaging agent in several tumors.
VCAM-1 scFv was labeled with 99mTc using succinimidyl 6-hydrazinium nicotinate hydrochloride, and 99mTc-HYNIC-VCAM-1scFv was successfully synthesized with a high radiolabeling yield.
VCAM-1 expression was evaluated in six cell lines by immunofluorescence staining.
In vitro binding assays showed different binding affinities of 99mTc-HYNIC-VCAM-1scFv in different tumor cell lines, with high uptake in B16F10 melanoma and HT1080 fibrosarcoma cells, which was consistent with immunofluorescence staining results.
In vivo SPECT planar imaging demonstrated that B16F10 and HT1080 tumors could be clearly visualized.
Less intense uptake was observed in human SKOV3.ip ovarian tumor, and weak uptake was observed in human A375m melanoma, MDA-MB-231 breast cancer, and 786-O renal tumors.
These findings were confirmed by biodistribution and immunofluorescence studies.
High uptake by B16F10 tumors was inhibited by excess unlabeled VCAM-1scFv.
99mTc-HYNIC-VCAM-1scFv, which selectively binds to VCAM-1, can provide a qualitative and semiquantitative method for noninvasive evaluation of VCAM-1 expression by tumors.
American Psychological Association (APA)
Zhang, Xiao& Hu, Fan& Liu, Chunbao& Yin, Lianglan& Zhang, Yingying& Zhang, Yongxue…[et al.]. 2018. Evaluation of 99mTc-HYNIC-VCAM-1scFv as a Potential Qualitative and Semiquantitative Probe Targeting Various Tumors. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1131539
Modern Language Association (MLA)
Zhang, Xiao…[et al.]. Evaluation of 99mTc-HYNIC-VCAM-1scFv as a Potential Qualitative and Semiquantitative Probe Targeting Various Tumors. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1131539
American Medical Association (AMA)
Zhang, Xiao& Hu, Fan& Liu, Chunbao& Yin, Lianglan& Zhang, Yingying& Zhang, Yongxue…[et al.]. Evaluation of 99mTc-HYNIC-VCAM-1scFv as a Potential Qualitative and Semiquantitative Probe Targeting Various Tumors. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1131539
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131539