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Imaging and Methotrexate Response Monitoring of Systemic Inflammation in Arthritic Rats Employing the Macrophage PET Tracer [18F]Fluoro-PEG-Folate
Joint Authors
Windhorst, Albert D.
Chandrupatla, Durga M. S. H.
Jansen, Gerrit
van der Laken, Conny J.
Molthoff, Carla F. M.
Mantel, Elise
Low, Philip S.
Matsuyama, Takami
Musters, René P.
Lammertsma, Adriaan Anthonius
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
In rheumatoid arthritis, articular inflammation is a hallmark of disease, while the involvement of extra-articular tissues is less well defined.
Here, we examined the feasibility of PET imaging with the macrophage tracer [18F]fluoro-PEG-folate, targeting folate receptor β (FRβ), to monitor systemic inflammatory disease in liver and spleen of arthritic rats before and after methotrexate (MTX) treatment.
Methods.
[18F]Fluoro-PEG-folate PET scans (60 min) were acquired in saline- and MTX-treated (1 mg/kg, 4x) arthritic rats, followed by tissue resection and radiotracer distribution analysis.
Liver and spleen tissues were stained for ED1/ED2-macrophage markers and FRβ expression.
Results.
[18F]Fluoro-PEG-folate PET and ex vivo tissue distribution studies revealed a significant (p<0.01) 2-fold lower tracer uptake in both liver and spleen of MTX-treated arthritic rats.
Consistently, ED1- and ED2-positive macrophages were significantly (p<0.01) decreased in liver (4-fold) and spleen (3-fold) of MTX-treated compared with saline-treated rats.
Additionally, FRβ-positive macrophages were also significantly reduced in liver (5-fold, p<0.005) and spleen (3-fold, p<0.01) of MTX- versus saline-treated rats.
Conclusions.
MTX treatment reduced activated macrophages in liver and spleen, as markers for systemic inflammation in these organs.
Macrophage PET imaging with [18F]fluoro-PEG-folate holds promise for detection of systemic inflammation in RA as well as therapy (MTX) response monitoring.
American Psychological Association (APA)
Chandrupatla, Durga M. S. H.& Jansen, Gerrit& Mantel, Elise& Low, Philip S.& Matsuyama, Takami& Musters, René P.…[et al.]. 2018. Imaging and Methotrexate Response Monitoring of Systemic Inflammation in Arthritic Rats Employing the Macrophage PET Tracer [18F]Fluoro-PEG-Folate. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1131557
Modern Language Association (MLA)
Chandrupatla, Durga M. S. H.…[et al.]. Imaging and Methotrexate Response Monitoring of Systemic Inflammation in Arthritic Rats Employing the Macrophage PET Tracer [18F]Fluoro-PEG-Folate. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1131557
American Medical Association (AMA)
Chandrupatla, Durga M. S. H.& Jansen, Gerrit& Mantel, Elise& Low, Philip S.& Matsuyama, Takami& Musters, René P.…[et al.]. Imaging and Methotrexate Response Monitoring of Systemic Inflammation in Arthritic Rats Employing the Macrophage PET Tracer [18F]Fluoro-PEG-Folate. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1131557
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131557