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Amyloid-Beta Radiotracer [18F]BF-227 Does Not Bind to Cytoplasmic Glial Inclusions of Postmortem Multiple System Atrophy Brain Tissue
Joint Authors
Zimmer, Luc
Billard, Thierry
Chauveau, Fabien
Verdurand, Mathieu
Levigoureux, Elise
Lancelot, Sophie
Zeinyeh, Waël
Quadrio, Isabelle
Perret-Liaudet, Armand
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-06
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The accumulation of aggregated alpha-synuclein (α-syn) in multiple brain regions is a neuropathological hallmark of synucleinopathies.
Multiple system atrophy (MSA) is a synucleinopathy characterized by the predominant cerebral accumulation of aggregated α-syn as cytoplasmic glial inclusions (CGI).
A premortem diagnosis tool would improve early diagnosis and help monitoring disease progression and therapeutic efficacy.
One Positron Emission Tomography (PET) study suggested [11C]BF-227 as a promising radiotracer for monitoring intracellular α-syn deposition in MSA patients.
We sought to confirm the binding of this radiotracer to α-syn using state-of-the-art autoradiography.
Medulla sections were obtained from 9 MSA patients and 9 controls (London Neurodegenerative Diseases Brain Bank).
[18F]BF-227, chemically identical to [11C]BF-227, was used at nanomolar concentrations to perform in vitro autoradiography assays.
Autoradiograms were superimposed on fluorescent staining from the conformational anti-α-syn antibody 5G4 and quantified after immunofluorescence-driven definition of regions of interest.
Autoradiography showed no specific signals in MSA patients in comparison to controls despite widespread pathology detected by immunofluorescence.
Autoradiography does not support a significant binding of [18F]BF-227 to CGI at concentrations typically achieved in PET experiments.
American Psychological Association (APA)
Verdurand, Mathieu& Levigoureux, Elise& Lancelot, Sophie& Zeinyeh, Waël& Billard, Thierry& Quadrio, Isabelle…[et al.]. 2018. Amyloid-Beta Radiotracer [18F]BF-227 Does Not Bind to Cytoplasmic Glial Inclusions of Postmortem Multiple System Atrophy Brain Tissue. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1131636
Modern Language Association (MLA)
Verdurand, Mathieu…[et al.]. Amyloid-Beta Radiotracer [18F]BF-227 Does Not Bind to Cytoplasmic Glial Inclusions of Postmortem Multiple System Atrophy Brain Tissue. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1131636
American Medical Association (AMA)
Verdurand, Mathieu& Levigoureux, Elise& Lancelot, Sophie& Zeinyeh, Waël& Billard, Thierry& Quadrio, Isabelle…[et al.]. Amyloid-Beta Radiotracer [18F]BF-227 Does Not Bind to Cytoplasmic Glial Inclusions of Postmortem Multiple System Atrophy Brain Tissue. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1131636
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1131636