A Novel Glucose Isomerase from Caldicellulosiruptor bescii with Great Potentials in the Production of High-Fructose Corn Syrup
Joint Authors
Dai, Chenxia
Miao, Tingting
Hai, Jinping
Xiao, Yunyi
Li, Ying
Zhao, Junren
Qiu, Hulin
Xu, Bo
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Glucose isomerase (GI) that catalyzes the conversion of D-glucose to D-fructose is one of the most important industrial enzymes for the production of high-fructose corn syrup (HFCS).
In this study, a novel GI (CbGI) was cloned from Caldicellulosiruptor bescii and expressed in Escherichia coli.
The purified recombinant CbGI (rCbGI) showed neutral and thermophilic properties.
It had optimal activities at pH 7.0 and 80°C and retained stability at 85°C.
In comparison with other reported GIs, rCbGI exhibited higher substrate affinity (Km=42.61 mM) and greater conversion efficiency (up to 57.3% with 3M D-glucose as the substrate).
The high catalytic efficiency and affinity of this CbGI is much valuable for the cost-effective production of HFCS.
American Psychological Association (APA)
Dai, Chenxia& Miao, Tingting& Hai, Jinping& Xiao, Yunyi& Li, Ying& Zhao, Junren…[et al.]. 2020. A Novel Glucose Isomerase from Caldicellulosiruptor bescii with Great Potentials in the Production of High-Fructose Corn Syrup. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132035
Modern Language Association (MLA)
Dai, Chenxia…[et al.]. A Novel Glucose Isomerase from Caldicellulosiruptor bescii with Great Potentials in the Production of High-Fructose Corn Syrup. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1132035
American Medical Association (AMA)
Dai, Chenxia& Miao, Tingting& Hai, Jinping& Xiao, Yunyi& Li, Ying& Zhao, Junren…[et al.]. A Novel Glucose Isomerase from Caldicellulosiruptor bescii with Great Potentials in the Production of High-Fructose Corn Syrup. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1132035
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1132035
