Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities
Joint Authors
Pham, Dinh-Chuong
Nguyen, Hoang-Chinh
Nguyen, Thanh-Hang Le
Ho, Hoang-Linh
Trinh, Thien-Kim
Riyaphan, Jirawat
Weng, Ching-Feng
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Celastrus hindsii is a potential source of flavonoids with biological activities.
This study aimed to develop an ultrasound-assisted technique for extracting flavonoids from leaves of C.
hindsii.
Response surface methodology was employed to optimize the extraction conditions for maximizing the total flavonoid content (TFC).
A maximum TFC of 23.6 mg QE/g was obtained under the extraction conditions of ultrasonic power of 130 W, extraction temperature of 40°C, extraction time of 29 min, and ethanol concentration of 65%.
The flavonoid-rich extracts were then studied for their antioxidant and anticancer activities.
The results showed that the C.
hindsii leaf extract exhibited potent radical scavenging activities against DPPH (IC50 of 164.85 μg/mL) and ABTS (IC50 of 89.05 μg/mL).
The extract also significantly inhibited the growth of 3 cancer cell lines MCF7, A549, and HeLa with the IC50 values of 88.1 μg/mL, 120.4 μg/mL, and 118.4 μg/mL, respectively.
Notably, the extract had no cytotoxicity effect on HK2 normal kidney cell line.
This study suggests that flavonoid-rich extract is a promising antioxidant and anticancer agent and that ultrasound-assisted extraction is an efficient method for extracting flavonoids from C.
hindsii leaves.
American Psychological Association (APA)
Pham, Dinh-Chuong& Nguyen, Hoang-Chinh& Nguyen, Thanh-Hang Le& Ho, Hoang-Linh& Trinh, Thien-Kim& Riyaphan, Jirawat…[et al.]. 2020. Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1133225
Modern Language Association (MLA)
Pham, Dinh-Chuong…[et al.]. Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1133225
American Medical Association (AMA)
Pham, Dinh-Chuong& Nguyen, Hoang-Chinh& Nguyen, Thanh-Hang Le& Ho, Hoang-Linh& Trinh, Thien-Kim& Riyaphan, Jirawat…[et al.]. Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1133225
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133225