No Significant Difference between Plasma miRNAs and Plasma-Derived Exosomal miRNAs from Healthy People
Joint Authors
Tian, Fei
Li, Rui
Chen, Zhenzhu
Shen, Yanting
Ge, Qinyu
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-01
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Background.
MicroRNAs (miRNAs) are small noncoding RNAs that are involved in many biological regulation processes.
Studies have reported that miRNAs are enriched in human plasma and plasma-derived exosome as novel diagnostic biomarkers.
The aim of this study was to determine whether the miRNA expression levels are different between plasma and plasma-derived exosome.
Methods.
We sequenced and quantified the miRNAs in plasma and exosome from healthy blood samples and validated three miRNAs in the two groups of lung cancer samples by qRT-PCR.
Results.
The sequencing results showed that only several of miRNAs were differential, while the qRT-PCR further validated that most of them did not have the consistent differences.
However, the levels of two upregulated miRNAs (miR-181b-5p and miR-21-5p) in lung cancer were significantly higher in exosomes than plasma.
Conclusions.
To the best of our knowledge, this study is the first to compare the expression levels of miRNAs between plasma and exosome in healthy blood samples.
Our data suggested that the miRNA levels were similar in the two parts of the healthy people, whereas the two onco-miRNAs were significantly enriched in the exosome of lung cancer patients.
American Psychological Association (APA)
Tian, Fei& Shen, Yanting& Chen, Zhenzhu& Li, Rui& Ge, Qinyu. 2017. No Significant Difference between Plasma miRNAs and Plasma-Derived Exosomal miRNAs from Healthy People. BioMed Research International،Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1133827
Modern Language Association (MLA)
Tian, Fei…[et al.]. No Significant Difference between Plasma miRNAs and Plasma-Derived Exosomal miRNAs from Healthy People. BioMed Research International No. 2017 (2017), pp.1-5.
https://search.emarefa.net/detail/BIM-1133827
American Medical Association (AMA)
Tian, Fei& Shen, Yanting& Chen, Zhenzhu& Li, Rui& Ge, Qinyu. No Significant Difference between Plasma miRNAs and Plasma-Derived Exosomal miRNAs from Healthy People. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1133827
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1133827