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Antiurolithiasis Activity of Bioactivity Guided Fraction of Bergenia ligulata against Ethylene Glycol Induced Renal Calculi in Rat
Joint Authors
Parveen, Rabea
Khan, Washim
Sharma, Ikshit
Alam, Md. Javed
Ahmad, Iftekhar
Ansari, Mohd Hafizur Rehman
Ahmad, Sayeed
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Dried rhizome of Bergenia ligulata (pashanbhed) is commonly used as a traditional herbal medicine with a wide range of therapeutic applications including urolithiasis.
Aqueous extract of B.
ligulata was prepared through maceration followed by decoction (mother extract, 35.9% w/w).
Further, polarity based fractions were prepared successively from mother extract which yielded 3.4, 2.9, 5.4, 7.5, and 11.3% w/w of hexane, toluene, dichloromethane (DCM), n-butanol, and water fractions, respectively.
The in vitro, ex vivo, and real-time antiurolithiasis activity of mother extract and fractions were carried out using aggregation assay in synthetic urine and in rat plasma.
The study revealed that DCM fraction has significantly (p<0.05) greater inhibitory potential than other fractions.
Ethylene glycol in drinking water (0.75%, v/v) for 28 days was used for induction of urolithiasis and the curative effects of mother extract and DCM fraction were checked for the level of oxalate, calcium, creatinine, uric acid, and urea of both urine and serum.
Treatment with mother extract and DCM fraction at a dose of 185 mg/kg and 7 mg/kg, respectively, in ethylene glycol induced rats resulted in a significant (p<0.05) decrease in serum and urine markers.
Histological study revealed lower number of calcium oxalate deposits with minimum damage in the kidneys of mother extract and DCM fraction treated rats.
This result provides a scientific basis for its traditional claims.
American Psychological Association (APA)
Sharma, Ikshit& Khan, Washim& Parveen, Rabea& Alam, Md. Javed& Ahmad, Iftekhar& Ansari, Mohd Hafizur Rehman…[et al.]. 2017. Antiurolithiasis Activity of Bioactivity Guided Fraction of Bergenia ligulata against Ethylene Glycol Induced Renal Calculi in Rat. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1134374
Modern Language Association (MLA)
Sharma, Ikshit…[et al.]. Antiurolithiasis Activity of Bioactivity Guided Fraction of Bergenia ligulata against Ethylene Glycol Induced Renal Calculi in Rat. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1134374
American Medical Association (AMA)
Sharma, Ikshit& Khan, Washim& Parveen, Rabea& Alam, Md. Javed& Ahmad, Iftekhar& Ansari, Mohd Hafizur Rehman…[et al.]. Antiurolithiasis Activity of Bioactivity Guided Fraction of Bergenia ligulata against Ethylene Glycol Induced Renal Calculi in Rat. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1134374
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134374