Cloning, Expression, and Immunogenicity of Fimbrial-F17A Subunit Vaccine against Escherichia coli Isolated from Bovine Mastitis
Joint Authors
Han, Bo
Ali, Tariq
Gao, Jian
Chen, Wei
Liu, Yongxia
Yin, Jinhua
Deng, Youtian
Zhang, Ju
Cheng, Jia
ur Rahman, Sadeeq
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
There is a need to identify and select new promising immunodominant antigens that have the ability to provide protective immunity against E.
coli causing bovine mastitis.
Recently we showed that f17a was found to be the most prevalent and crucial virulent factor among the pathogenic E.
coli isolated from bovine mastitis.
Here, in this report, the recombinant F17A based subunit vaccine adjuvant with MF59 was tested for immunogenicity against E.
coli in a murine model.
The vaccinated mice did not show any abnormal behavioral changes and histopathological lesions after vaccination.
The specific antibody level against F17A was significantly higher in MF59-adjuvant-group, and also lasted for longer duration with a significant (P<0.01) production level of IgG1 and IgG2a.
Moreover, we noted higher survival rate in mice injected with F17A-MF59-adjuvant group after challenging with the clinical E.
coli strain.
Our findings of bacterial clearance test revealed that elimination rate from liver, spleen, and kidney in MF59-adjuvant-group was significantly higher than the control group.
Finally, the proportion of CD4+T cells was increased, while CD8+ was decreased in MF59-adjuvant group.
In conclusion, the current study reveals the capability of F17A-MF59 as a potential vaccine candidate against pathogenic E.
coli causing mastitis in dairy animals.
American Psychological Association (APA)
Chen, Wei& Liu, Yongxia& Yin, Jinhua& Deng, Youtian& Ali, Tariq& Zhang, Ju…[et al.]. 2017. Cloning, Expression, and Immunogenicity of Fimbrial-F17A Subunit Vaccine against Escherichia coli Isolated from Bovine Mastitis. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1135865
Modern Language Association (MLA)
Chen, Wei…[et al.]. Cloning, Expression, and Immunogenicity of Fimbrial-F17A Subunit Vaccine against Escherichia coli Isolated from Bovine Mastitis. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1135865
American Medical Association (AMA)
Chen, Wei& Liu, Yongxia& Yin, Jinhua& Deng, Youtian& Ali, Tariq& Zhang, Ju…[et al.]. Cloning, Expression, and Immunogenicity of Fimbrial-F17A Subunit Vaccine against Escherichia coli Isolated from Bovine Mastitis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1135865
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135865