From Human Mesenchymal Stem Cells to Insulin-Producing Cells: Comparison between Bone Marrow- and Adipose Tissue-Derived Cells
Joint Authors
Ghoneim, Mohamed A.
Khater, Sherry M.
Abdel-Rahman, Engy A.
Ali, Sameh S.
Reda, Asmaa M.
Ismail, Hossam El-Din A.
Ashamallah, Sylvia A.
Ismail, Amani M.
Gabr, Mahmoud M.
Zakaria, Mahmoud M.
El-Badri, Nagwa
Refaie, Ayman F.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The aim of this study is to compare human bone marrow-derived mesenchymal stem cells (BM-MSCs) and adipose tissue-derived mesenchymal stem cells (AT-MSCs), for their differentiation potentials to form insulin-producing cells.
BM-MSCs were obtained during elective orthotopic surgery and AT-MSCs from fatty aspirates during elective cosmetics procedures.
Following their expansion, cells were characterized by phenotyping, trilineage differentiation ability, and basal gene expression of pluripotency genes and for their metabolic characteristics.
Cells were differentiated according to a Trichostatin-A based protocol.
The differentiated cells were evaluated by immunocytochemistry staining for insulin and c-peptide.
In addition the expression of relevant pancreatic endocrine genes was determined.
The release of insulin and c-peptide in response to a glucose challenge was also quantitated.
There were some differences in basal gene expression and metabolic characteristics.
After differentiation the proportion of the resulting insulin-producing cells (IPCs), was comparable among both cell sources.
Again, there were no differences neither in the levels of gene expression nor in the amounts of insulin and c-peptide release as a function of glucose challenge.
The properties, availability, and abundance of AT-MSCs render them well-suited for applications in regenerative medicine.
Conclusion.
BM-MSCs and AT-MSCs are comparable regarding their differential potential to form IPCs.
The availability and properties of AT-MSCs render them well-suited for applications in regenerative medicine.
American Psychological Association (APA)
Gabr, Mahmoud M.& Zakaria, Mahmoud M.& Refaie, Ayman F.& Abdel-Rahman, Engy A.& Reda, Asmaa M.& Ali, Sameh S.…[et al.]. 2017. From Human Mesenchymal Stem Cells to Insulin-Producing Cells: Comparison between Bone Marrow- and Adipose Tissue-Derived Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1136461
Modern Language Association (MLA)
Gabr, Mahmoud M.…[et al.]. From Human Mesenchymal Stem Cells to Insulin-Producing Cells: Comparison between Bone Marrow- and Adipose Tissue-Derived Cells. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1136461
American Medical Association (AMA)
Gabr, Mahmoud M.& Zakaria, Mahmoud M.& Refaie, Ayman F.& Abdel-Rahman, Engy A.& Reda, Asmaa M.& Ali, Sameh S.…[et al.]. From Human Mesenchymal Stem Cells to Insulin-Producing Cells: Comparison between Bone Marrow- and Adipose Tissue-Derived Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1136461
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136461