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The Use of Xanthan Gum as Vaccine Adjuvant: An Evaluation of Immunostimulatory Potential in BALBc Mice and Cytotoxicity In Vitro
Joint Authors
Hartwig, Daiane D.
Oliveira, Thaís Larré
Collares, Thaís Farias
Monte, Leonardo Garcia
Inda, Guilherme Roig
Vendrusculo, Claire T.
Schuch, Rodrigo
Dellagostin, Odir A.
Moreira, Angelita da S.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The successful production of new, safe, and effective vaccines that generate immunological memory is directly related to adjuvant feature, which is responsible for increasing and/or modulating the immune response.
Several compounds display adjuvant activity, including carbohydrates.
These compounds play important roles in the immune response, as well as having biocompatible properties in vaccine formulations.
One such carbohydrate is xanthan gum, a polysaccharide that is produced by the plant-pathogenic bacterium Xanthomonas spp., which has adjuvant attributes.
This study evaluated the immune response induced by xanthan gum associated with ovalbumin in BALB/c mice, which were subcutaneously immunized, in terms of antibody production (IgG1, IgG2a, IgG2b, and IgG3), and assessed the levels of IFN-γ in the splenocyte culture using indirect ELISA.
Furthermore, we investigated in vitro cytotoxicity of xanthan in the embryo fibroblasts cell line of the NIH/3T3 mouse by MTT assay and propidium iodide uptake assay.
The mice immunized with ovalbumin plus xanthan gum exhibited higher antibody IgG1 responses than control groups.
Furthermore, the xanthan polysaccharide was capable of increasing the immunogenicity of antigens by producing IFN-γ and did not exhibit cytotoxicity effects in NIH/3T3 mouse fibroblast cells, considered a promising candidate for vaccine adjuvant.
American Psychological Association (APA)
Schuch, Rodrigo& Oliveira, Thaís Larré& Collares, Thaís Farias& Monte, Leonardo Garcia& Inda, Guilherme Roig& Dellagostin, Odir A.…[et al.]. 2017. The Use of Xanthan Gum as Vaccine Adjuvant: An Evaluation of Immunostimulatory Potential in BALBc Mice and Cytotoxicity In Vitro. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1136564
Modern Language Association (MLA)
Schuch, Rodrigo…[et al.]. The Use of Xanthan Gum as Vaccine Adjuvant: An Evaluation of Immunostimulatory Potential in BALBc Mice and Cytotoxicity In Vitro. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1136564
American Medical Association (AMA)
Schuch, Rodrigo& Oliveira, Thaís Larré& Collares, Thaís Farias& Monte, Leonardo Garcia& Inda, Guilherme Roig& Dellagostin, Odir A.…[et al.]. The Use of Xanthan Gum as Vaccine Adjuvant: An Evaluation of Immunostimulatory Potential in BALBc Mice and Cytotoxicity In Vitro. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1136564
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136564