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Characterization of Exopolysaccharide Produced by Streptococcus thermophilus CC30
Joint Authors
Kanamarlapudi, Sri Lakshmi Ramya Krishna
Muddada, Sudhamani
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
An exopolysaccharide (EPS) producing strain CC30 was isolated from raw milk and identified as Streptococcus thermophilus with morphological and 16S sequencing analysis.
The strain was shown to produce 1.95 g/L of EPS when grown in skim milk lactose medium at 30°C by increasing the viscosity of the medium.
The EPS was isolated and purified, and it was shown to consist of glucose and galactose in 1 : 1 ratio, with molecular weights ranging from 58 to 180 kDa.
FTIR spectroscopy indicated the EPS to have amide, hydroxyl, and carboxyl groups.
Under Atomic Force Microscopy, EPS showed spike-like lumps of EPS.
Scanning Electron Microscopy (SEM) studies showed that it had irregular lumps with a coarse surface.
The EPS displayed pseudoplastic nature.
Thermogravimetric analysis (TGA) reported a degradation temperature of 110.84°C.
The purified EPS exhibited reducing activity, hydrogen peroxide radical scavenging activity, and emulsification activity.
The results of the present study indicated that EPS producing Streptococcus thermophilus could serve as a promising candidate for further exploitation in food industry.
American Psychological Association (APA)
Kanamarlapudi, Sri Lakshmi Ramya Krishna& Muddada, Sudhamani. 2017. Characterization of Exopolysaccharide Produced by Streptococcus thermophilus CC30. BioMed Research International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1136853
Modern Language Association (MLA)
Kanamarlapudi, Sri Lakshmi Ramya Krishna& Muddada, Sudhamani. Characterization of Exopolysaccharide Produced by Streptococcus thermophilus CC30. BioMed Research International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1136853
American Medical Association (AMA)
Kanamarlapudi, Sri Lakshmi Ramya Krishna& Muddada, Sudhamani. Characterization of Exopolysaccharide Produced by Streptococcus thermophilus CC30. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1136853
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136853