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Mechanism of Reduced Susceptibility to Fosfomycin in Escherichia coli Clinical Isolates
Joint Authors
Yokota, Shin-ichi
Ohkoshi, Yasuo
Sato, Toyotaka
Suzuki, Yuuki
Yamamoto, Soh
Shiraishi, Tsukasa
Ogasawara, Noriko
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In recent years, multidrug resistance of Escherichia coli has become a serious problem.
However, resistance to fosfomycin (FOM) has been low.
We screened E.
coli clinical isolates with reduced susceptibility to FOM and characterized molecular mechanisms of resistance and reduced susceptibility of these strains.
Ten strains showing reduced FOM susceptibility (MIC ≥ 8 μg/mL) in 211 clinical isolates were found and examined.
Acquisition of genes encoding FOM-modifying enzyme genes (fos genes) and mutations in murA that underlie high resistance to FOM were not observed.
We examined ability of FOM incorporation via glucose-6-phosphate (G6P) transporter and sn-glycerol-3-phosphate transporter.
In ten strains, nine showed lack of growth on M9 minimum salt agar supplemented with G6P.
Eight of the ten strains showed fluctuated induction by G6P of uhpT that encodes G6P transporter expression.
Nucleotide sequences of the uhpT, uhpA, glpT, ptsI, and cyaA shared several deletions and amino acid mutations in the nine strains with lack of growth on G6P-supplemented M9 agar.
In conclusion, reduction of uhpT function is largely responsible for the reduced sensitivity to FOM in clinical isolates that have not acquired FOM-modifying genes or mutations in murA.
However, there are a few strains whose mechanisms of reduced susceptibility to FOM are still unclear.
American Psychological Association (APA)
Ohkoshi, Yasuo& Sato, Toyotaka& Suzuki, Yuuki& Yamamoto, Soh& Shiraishi, Tsukasa& Ogasawara, Noriko…[et al.]. 2017. Mechanism of Reduced Susceptibility to Fosfomycin in Escherichia coli Clinical Isolates. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137645
Modern Language Association (MLA)
Ohkoshi, Yasuo…[et al.]. Mechanism of Reduced Susceptibility to Fosfomycin in Escherichia coli Clinical Isolates. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1137645
American Medical Association (AMA)
Ohkoshi, Yasuo& Sato, Toyotaka& Suzuki, Yuuki& Yamamoto, Soh& Shiraishi, Tsukasa& Ogasawara, Noriko…[et al.]. Mechanism of Reduced Susceptibility to Fosfomycin in Escherichia coli Clinical Isolates. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1137645
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137645