Application of a New Type of Natural Calcined Bone Repair Material Combined with Concentrated Growth Factors in Bone Regeneration in Rabbit Critical-Sized Calvarial Defect

Abstract EN

Purpose.

This study is aimed at investigating bone regeneration in critical-sized defects in rabbit calvarium using a novel nano- (n-) hydroxyapatite hybrid scaffold with concentrated growth factors (CGFs).

Methods.

Twenty-four male adult rabbits were chosen to establish a critical-sized bone defect model and randomly divided into two groups.

Two defects of 15 mm diameter each were created in the parietal bone of each animal.

Group A had n-hydroxyapatite hybrid scaffold placed in the experimental defect on the right, and the left defect was unfilled as blank.

Group B had hydroxyapatite hybrid scaffold mixed with CGF placed in the right defect and CGF on the left.

Six animals in each group were sacrificed after 6 and 12 weeks.

Cone-beam computed tomography system scanning and hematoxylin and eosin (HE) staining were used to detect osteogenesis within the defects.

Results.

The treatment with n-hydroxyapatite hybrid scaffold along with CGF resulted in a significantly higher amount of new bone at 6 and 12 weeks compared to the treatment with CGF alone and the controls.

No apparent inflammation and foreign body reaction were observed through HE staining.

Conclusions.

The new synthesized n-hydroxyapatite hybrid scaffold and CGF can be applied for bone defect regeneration to promote the process to a certain extent.