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The Platelet microRNA Profile of Kawasaki Disease: Identification of Novel Diagnostic Biomarkers
Joint Authors
Ding, Guohui
Zhang, Hong
Ning, Qianqian
Chen, Liqin
Song, Sirui
Xu, Kangping
Liu, Jia
Zhou, Yiwen
Zang, Chenyang
Li, Guang
Chen, Feng
Jia, Jia
Huang, Min
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-18
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Challenging diagnosis and unknown etiology of Kawasaki disease (KD) increase the coronary artery lesions incidence.
microRNAs (miRNAs) are the most promising biomarkers because of their stability in peripheral blood and noninvasive measurement procedure, whose potential utility have been proved in cancers.
To explore the utility of differentially expressed (DE) miRNAs as early diagnostic markers, 44 patients (25 incomplete KD and 19 complete KD) and 31 febrile controls were recruited for small RNA sequencing.
From all the 1922 expressed miRNA, 210 DE miRNAs were found between KD and febrile control groups.
Though platelet miRNA profiles of complete KD incomplete KD were much similar through cluster analysis, the DE miRNAs were not identical.
Eight DE miRNAs were validated by real-time quantitative PCR (qRT-PCR) in complete or incomplete KD groups using a normalizer, miR-126-3p, which was identified by geNorm and NormFinder tools.
The expression level of miRNAs continuous changed over time was observed and the function analysis showed the potential role of miRNAs as therapeutic biomarkers.
Additionally, the prediction model for KD showed a sensitivity of 78.8% and a specificity of 71.4%, respectively.
This study used small RNA sequencing to identify miRNA biomarkers KD diagnosis based on a large sample size.
Our findings shine a light on the understanding of molecular pathogenesis of KD and may improve the accuracy of KD diagnosis and prognosis in clinical.
American Psychological Association (APA)
Ning, Qianqian& Chen, Liqin& Song, Sirui& Zhang, Hong& Xu, Kangping& Liu, Jia…[et al.]. 2020. The Platelet microRNA Profile of Kawasaki Disease: Identification of Novel Diagnostic Biomarkers. BioMed Research International،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1137938
Modern Language Association (MLA)
Ning, Qianqian…[et al.]. The Platelet microRNA Profile of Kawasaki Disease: Identification of Novel Diagnostic Biomarkers. BioMed Research International No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1137938
American Medical Association (AMA)
Ning, Qianqian& Chen, Liqin& Song, Sirui& Zhang, Hong& Xu, Kangping& Liu, Jia…[et al.]. The Platelet microRNA Profile of Kawasaki Disease: Identification of Novel Diagnostic Biomarkers. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1137938
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1137938