Quantitative Serum Proteomic Analysis of Essential Hypertension Using iTRAQ Technique
Joint Authors
Jiang, Haiqiang
Xu, Jing-Wen
Li, Yun-Lun
Zhang, Shi-Jun
Yang, Wen-Qing
Nie, Wen-Ting
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-22
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Essential hypertension (EH) is a risk factor for some severe diseases.
This study aimed to screen out serum special proteins and seek interaction between them, which would provide new therapeutic targets and elucidate the comprehensive pathophysiological mechanism for EH.
Patients with EH (Group A, n=47) and healthy controls (HC) (Group B, n=47) were recruited in this study.
Serums from the two groups were analyzed with isobaric tags for relative and absolute quantitation coupled two-dimensional liquid chromatography followed by electrospray ionization-tandem mass spectrometry technique, while the candidate special proteins were verified with ELISA and western blot.
A total of 404 proteins were identified, of which 30 proteins were upregulated (>1.2-fold, p<0.05) and 81 proteins were downregulated (<0.833-fold, p<0.05) compared with HC group.
With GO, KEGG analysis, and literature retrieval, 4 proteins, cathepsin G, transforming growth factor beta-1, hyaluronidase-1, and kininogen-1, were found jointly involved in the renin-angiotensin-aldosterone system and kallikrein-kinin system.
The profiles of these 4 candidate proteins were confirmed with ELISA and western blot.
The concentration variation of these 4 proteins could better predict the occurrence and illustrate the pathophysiological mechanism of EH.
And their discovery may help pave the way for exploring new therapies of EH.
American Psychological Association (APA)
Xu, Jing-Wen& Li, Yun-Lun& Zhang, Shi-Jun& Yang, Wen-Qing& Nie, Wen-Ting& Jiang, Haiqiang. 2017. Quantitative Serum Proteomic Analysis of Essential Hypertension Using iTRAQ Technique. BioMed Research International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1138211
Modern Language Association (MLA)
Xu, Jing-Wen…[et al.]. Quantitative Serum Proteomic Analysis of Essential Hypertension Using iTRAQ Technique. BioMed Research International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1138211
American Medical Association (AMA)
Xu, Jing-Wen& Li, Yun-Lun& Zhang, Shi-Jun& Yang, Wen-Qing& Nie, Wen-Ting& Jiang, Haiqiang. Quantitative Serum Proteomic Analysis of Essential Hypertension Using iTRAQ Technique. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1138211
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138211