99mTc-68Ga-ICG-Labelled Macroaggregates and Nanocolloids of Human Serum Albumin: Synthesis Procedures of a Trimodal Imaging Agent Using Commercial Kits
Joint Authors
Persico, Marco Giovanni
Marenco, Manuela
De Matteis, Gianluca
Manfrinato, Giulia
Cavenaghi, Giorgio
Sgarella, Adele
Aprile, Carlo
Lodola, Lorenzo
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Recent developments in sentinel lymph node (SLN) and radio occult lesion localization (ROLL) highlight the need for a multimodal contrast agent, providing better presurgical PET imaging and improved intraoperative mapping thanks to fluorescence detection.
For this reason, we have studied a trimodal SLN/ROLL targeting agent (99mTc-68Ga-ICG) with commercially available kits of macroaggregated or nanocolloidal albumin (MA/NC-HSA).
68Ga PET imaging does provide better spatial resolution and makes it possible to predict signal intensity during surgery.
The presence of 99mTc assesses the efficacy of these compounds in vitro and also during the surgery procedure.
The aim of this study was to optimise the labelling and tagging of these two radiopharmaceuticals and assess their yields and stability.
Kits of MA/NC-HSA particles (Pulmocis® and NanoAlbumon®) were used for sequential radiolabelling with 99mTc and 68Ga.
Fluorescent tagging was performed using indocyanine green, a tricarbocyanine dye.
The ITLC radiochemical purity of the trilabelled MA/NC-HSA was >95%.
Fluorescent purity was measured by scanning the strips with a PhotoDynamicEye probe.
Finally, in vitro stability tests, performed with DTPA and human serum solutions, assessed the efficacy of fluorescent tagging and radiolabelling.
American Psychological Association (APA)
Persico, Marco Giovanni& Marenco, Manuela& De Matteis, Gianluca& Manfrinato, Giulia& Cavenaghi, Giorgio& Sgarella, Adele…[et al.]. 2020. 99mTc-68Ga-ICG-Labelled Macroaggregates and Nanocolloids of Human Serum Albumin: Synthesis Procedures of a Trimodal Imaging Agent Using Commercial Kits. Contrast Media & Molecular Imaging،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1139242
Modern Language Association (MLA)
Persico, Marco Giovanni…[et al.]. 99mTc-68Ga-ICG-Labelled Macroaggregates and Nanocolloids of Human Serum Albumin: Synthesis Procedures of a Trimodal Imaging Agent Using Commercial Kits. Contrast Media & Molecular Imaging No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1139242
American Medical Association (AMA)
Persico, Marco Giovanni& Marenco, Manuela& De Matteis, Gianluca& Manfrinato, Giulia& Cavenaghi, Giorgio& Sgarella, Adele…[et al.]. 99mTc-68Ga-ICG-Labelled Macroaggregates and Nanocolloids of Human Serum Albumin: Synthesis Procedures of a Trimodal Imaging Agent Using Commercial Kits. Contrast Media & Molecular Imaging. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1139242
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139242