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The Optimal Timing for Pancreatic Islet Transplantation into Subcutaneous Scaffolds Assessed by Multimodal Imaging
Joint Authors
Berkova, Zuzana
Kriz, Jan
Fabryova, Eva
Kosinova, Lucie
Gálisová, Andrea
Sticová, Eva
Jirátová, Markéta
Herynek, Vít
Hájek, Milan
Jirák, Daniel
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-26
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Subcutaneously implanted polymeric scaffolds represent an alternative transplantation site for pancreatic islets (PIs) with the option of vascularisation enhancement by mesenchymal stem cells (MSC).
Nevertheless, a proper timing of the transplantation steps is crucial.
In this study, scaffolds supplemented with plastic rods were implanted into diabetic rats and two timing schemes for subsequent transplantation of bioluminescent PIs (4 or 7 days after rod removal) were examined by multimodal imaging.
The cavities were left to heal spontaneously or with 10 million injected MSCs.
Morphological and vascularisation changes were examined by MRI, while the localisation and viability of transplanted islets were monitored by bioluminescence imaging.
The results show that PIs transplanted 4 days after rod removal showed the higher optical signal and vascularisation compared to transplantation after 7 days.
MSCs slightly improved vascularisation of the graft but hindered therapeutic efficiency of PIs.
Long-term glycaemia normalisation (4 months) was attained in 80% of animals.
In summary, multimodal imaging confirmed the long-term survival and function of transplanted PIs in the devices.
The best outcome was reached with PIs transplanted on day 4 after rod removal and therefore the suggested protocol holds a potential for further applications.
American Psychological Association (APA)
Gálisová, Andrea& Fabryova, Eva& Sticová, Eva& Kosinova, Lucie& Jirátová, Markéta& Herynek, Vít…[et al.]. 2017. The Optimal Timing for Pancreatic Islet Transplantation into Subcutaneous Scaffolds Assessed by Multimodal Imaging. Contrast Media & Molecular Imaging،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1141778
Modern Language Association (MLA)
Gálisová, Andrea…[et al.]. The Optimal Timing for Pancreatic Islet Transplantation into Subcutaneous Scaffolds Assessed by Multimodal Imaging. Contrast Media & Molecular Imaging No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1141778
American Medical Association (AMA)
Gálisová, Andrea& Fabryova, Eva& Sticová, Eva& Kosinova, Lucie& Jirátová, Markéta& Herynek, Vít…[et al.]. The Optimal Timing for Pancreatic Islet Transplantation into Subcutaneous Scaffolds Assessed by Multimodal Imaging. Contrast Media & Molecular Imaging. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1141778
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1141778