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Piper cubeba L. Methanol Extract Has Anti-Inflammatory Activity Targeting SrcSyk via NF-κB Inhibition
Joint Authors
Qomaladewi, Nurinanda Prisky
Aziz, Nur
Kim, Mi-Yeon
Cho, Jae Youl
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Piper cubeba L.
is a plant in the Piperaceae family that is generally found in tropical countries and acts as an antioxidant and anti-inflammatory agent.
Unfortunately, the molecular mechanism of the anti-inflammatory activity has not been fully investigated.
In this study, we elucidated the anti-inflammatory mechanism by focusing on NF-κB signaling, which is considered a prototypical inflammatory signaling pathway in both innate and adaptive immune functions.
Cellular activity and the molecular target of Pc-ME were identified in macrophage RAW264.7 cells and HEK293T cells by assessing NO production, cytokine expression by RT-PCR, luciferase gene reporter assay, and protein regulation in cytoplasm by Western blot upon NF-κB activation.
Pc-ME reduced NO production without any cell toxicity; inhibited expression of proinflammatory cytokines such as iNOS and IL-6; downregulated NF-κB activation mediated by both MyD88 and TRIF; and diminished the phosphorylation of IκBα, IKKα/β, Akt, p85, Src, and Syk.
Pc-ME inhibited Syk and Src autophosphorylation during overexpression in HEK cells, which confirmed our hypothesis that Syk and Src were signaling targets of Pc-ME.
These findings indicate that Piper cubeba L.
has anti-inflammatory activity by targeting Src/Syk in the NF-κB pathway.
American Psychological Association (APA)
Qomaladewi, Nurinanda Prisky& Aziz, Nur& Kim, Mi-Yeon& Cho, Jae Youl. 2019. Piper cubeba L. Methanol Extract Has Anti-Inflammatory Activity Targeting SrcSyk via NF-κB Inhibition. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1148613
Modern Language Association (MLA)
Qomaladewi, Nurinanda Prisky…[et al.]. Piper cubeba L. Methanol Extract Has Anti-Inflammatory Activity Targeting SrcSyk via NF-κB Inhibition. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1148613
American Medical Association (AMA)
Qomaladewi, Nurinanda Prisky& Aziz, Nur& Kim, Mi-Yeon& Cho, Jae Youl. Piper cubeba L. Methanol Extract Has Anti-Inflammatory Activity Targeting SrcSyk via NF-κB Inhibition. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1148613
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1148613