Investigation of the Optimum Preparation of Peach Gum Polysaccharides and the In Vivo and In Vitro Therapeutic Effects on Acute Pyelonephritis
Joint Authors
Yang, Guangzhong
Zhang, Feifei
Bai, Jie
Zheng, Yao
Liang, Shuai
Lei, Lei
Deng, Xin
Li, Weijun
Liu, Peng
Ren, Yongshen
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-12
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Background.
Acute pyelonephritis (APN), known as stranguria in traditional Chinese medicine, is commonly treated with antibiotics.
However, the rise in antibiotic resistance and the high rates of recurrence of APN make its treatment complicated, thus the development of alternative therapies is critical.
Peach gum has long been recognized by traditional Chinese medicine as a food with medicinal value of relieving stranguria, but whether and how its primary constituent peach gum polysaccharides (PGPs) contribute to the diuretic function is still not clear.
Purpose.
The aim of this study was to investigate the optimum extraction process of PGPs and to evaluate its therapeutic effect on APN rats and to discover the underlying mechanism.
Methods.
In this study, surface design optimization was adopted to optimize the preparation of PGPs and HPLC and FT-IR spectra were used to evaluate the quality of PGPs; APN model rat was established by the Escherichia coli urinary tract infection method; the therapeutic effect and mechanism of PGPs on APN were determined by the visceral index, biochemical indicators, pathological section of the APN rat, and diuretic activity on mice and antibacterial activity in vitro.
Results.
Compared with an untreated APN group, the results showed that treatment with PGPs increased the APN-induced attenuation of secretory immunoglobulin A (sIgA) and creatinine clearance and decreased the APN-induced enhancement of the number of white blood cell (WBC), neutrophil counts (NC), bacteria load of the kidneys, kidney index, serum creatinine, urine volume, blood urea nitrogen (BUN), and interleukin-2 (IL-2) levels.
The mechanism underlying these effects was further elucidated through in vitro experiments of the antibacterial and antiadhesion effects of PGPs.
Conclusion.
Due to the good therapeutic effects and advantages of PGPs, it could be considered as an alternative medicine to treat APN.
American Psychological Association (APA)
Zhang, Feifei& Bai, Jie& Zheng, Yao& Liang, Shuai& Lei, Lei& Deng, Xin…[et al.]. 2019. Investigation of the Optimum Preparation of Peach Gum Polysaccharides and the In Vivo and In Vitro Therapeutic Effects on Acute Pyelonephritis. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1149101
Modern Language Association (MLA)
Zhang, Feifei…[et al.]. Investigation of the Optimum Preparation of Peach Gum Polysaccharides and the In Vivo and In Vitro Therapeutic Effects on Acute Pyelonephritis. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-19.
https://search.emarefa.net/detail/BIM-1149101
American Medical Association (AMA)
Zhang, Feifei& Bai, Jie& Zheng, Yao& Liang, Shuai& Lei, Lei& Deng, Xin…[et al.]. Investigation of the Optimum Preparation of Peach Gum Polysaccharides and the In Vivo and In Vitro Therapeutic Effects on Acute Pyelonephritis. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1149101
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149101