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Nimbolide Represses the Proliferation, Migration, and Invasion of Bladder Carcinoma Cells via Chk2-Mediated G2M Phase Cell Cycle Arrest, Altered Signaling Pathways, and Reduced Transcription Factors-Associated MMP-9 Expression
Joint Authors
Kim, Wun-Jae
Kim, Gonhyung
Shin, Seung-Shick
Hwang, Byungdoo
Muhammad, Kashif
Gho, Yujeong
Song, Jun-Hui
Moon, Sung-Kwon
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-14
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Nimbolide, an active chemical constituent of Azadirachta indica, reportedly has several physiological effects.
Here, we assessed novel anticancer effects of nimbolide against bladder cancer EJ and 5637 cells.
Nimbolide treatment inhibited the proliferation of both bladder cancer cell lines with an IC50 value of 3 μM.
Treatment of cells with nimbolide induced G2/M phase cell cycle arrest via both Chk2-Cdc25C-Cdc2/cyclin B1-Wee1 pathway and Chk2-p21WAF1-Cdc2/cyclin B1-Wee1 pathway.
Nimbolide increased JNK phosphorylation and decreased p38MAPK and AKT phosphorylation.
Additionally, nimbolide impeded both wound healing migration and invasion abilities by suppressing matrix metalloproteinase-9 (MMP-9) activity.
Finally, nimbolide repressed the binding activity of NF-κB, Sp-1, and AP-1 motifs, which are key transcription factors for MMP-9 activity regulation.
Overall, our study indicates that nimbolide is a potential chemotherapeutic agent for bladder cancer.
American Psychological Association (APA)
Shin, Seung-Shick& Hwang, Byungdoo& Muhammad, Kashif& Gho, Yujeong& Song, Jun-Hui& Kim, Wun-Jae…[et al.]. 2019. Nimbolide Represses the Proliferation, Migration, and Invasion of Bladder Carcinoma Cells via Chk2-Mediated G2M Phase Cell Cycle Arrest, Altered Signaling Pathways, and Reduced Transcription Factors-Associated MMP-9 Expression. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1149568
Modern Language Association (MLA)
Shin, Seung-Shick…[et al.]. Nimbolide Represses the Proliferation, Migration, and Invasion of Bladder Carcinoma Cells via Chk2-Mediated G2M Phase Cell Cycle Arrest, Altered Signaling Pathways, and Reduced Transcription Factors-Associated MMP-9 Expression. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1149568
American Medical Association (AMA)
Shin, Seung-Shick& Hwang, Byungdoo& Muhammad, Kashif& Gho, Yujeong& Song, Jun-Hui& Kim, Wun-Jae…[et al.]. Nimbolide Represses the Proliferation, Migration, and Invasion of Bladder Carcinoma Cells via Chk2-Mediated G2M Phase Cell Cycle Arrest, Altered Signaling Pathways, and Reduced Transcription Factors-Associated MMP-9 Expression. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1149568
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149568