Antioxidant Properties of Unripe Carica papaya Fruit Extract and Its Protective Effects against Endothelial Oxidative Stress
Joint Authors
Wattanapitayakul, Suvara K.
Jarisarapurin, Wattanased
Sanrattana, Wariya
Kunchana, Khwandow
Chularojmontri, Linda
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-20
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
It has been proven that high consumption of fruit and vegetable lowers the risks of cardiovascular and other oxidative stress-related diseases.
Here we evaluated the effects of a tropical fruit, unripe Carica papaya (UCP), on endothelial protection against oxidative damage induced by H2O2.
The antioxidant properties of UCP were investigated using the assays of FRAP and ORAC and specific ROS scavenging activities (H2O2, O 2 • - , OH•, HOCl).
Cytoprotective property was tested in human endothelial cell line EA.hy926 with respect to cell survival, intracellular ROS levels, antioxidant enzyme activities (CAT, SOD, GPX), survival/stress signaling (AKT, JNK, p38), and nuclear signaling (Nrf2, NF-kB).
UCP processed high antioxidant activity and scavenging activity against H2O2> OH•> O 2 • - > HOCl, respectively.
UCP improved cell survival in the milieu of ROS reduction.
While SOD was increased by UCP, CAT activity was enhanced when cells were challenged with H2O2.
UCP had no impact on H2O2-activated AKT, JNK, and p38 signaling but significantly decreased nuclear NF-κB levels.
The overactivation of Nrf2 in response to oxidative stress was constrained by UCP.
In conclusion, UCP protected endothelial cells against oxidative damage through intracellular ROS reduction, enhanced CAT activity, suppression of NF-kB, and prohibition of Nrf2 dysregulation.
Thus, UCP might be a candidate for development of nutraceuticals against CVD and oxidative-related diseases and conditions.
American Psychological Association (APA)
Jarisarapurin, Wattanased& Sanrattana, Wariya& Chularojmontri, Linda& Kunchana, Khwandow& Wattanapitayakul, Suvara K.. 2019. Antioxidant Properties of Unripe Carica papaya Fruit Extract and Its Protective Effects against Endothelial Oxidative Stress. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1149949
Modern Language Association (MLA)
Jarisarapurin, Wattanased…[et al.]. Antioxidant Properties of Unripe Carica papaya Fruit Extract and Its Protective Effects against Endothelial Oxidative Stress. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1149949
American Medical Association (AMA)
Jarisarapurin, Wattanased& Sanrattana, Wariya& Chularojmontri, Linda& Kunchana, Khwandow& Wattanapitayakul, Suvara K.. Antioxidant Properties of Unripe Carica papaya Fruit Extract and Its Protective Effects against Endothelial Oxidative Stress. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1149949
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1149949