Within-Subject Reliability and between-Subject Variability of Oxidative Stress Markers in Saliva of Healthy Subjects: A Longitudinal Pilot Study

Joint Authors

Andabak Rogulj, Ana
Alajbeg, Iva Z.
Lapić, Ivana
Rogić, Dunja
Vuletić, Lea
Illeš, Davor
Knezović Zlatarić, Dubravka
Badel, Tomislav
Vrbanović, Ema
Alajbeg, Ivan

Source

Disease Markers

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-11-15

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Diseases

Abstract EN

The present study evaluated diurnal variations and day-to-day fluctuations of salivary oxidative stress (OS) markers in healthy adult individuals.

Whole unstimulated saliva was collected at 2 time intervals over 3 consecutive days.

Glutathione peroxidase (GPX), superoxide dismutase (SOD), total antioxidant capacity (TAC), and uric acid (UA) were analyzed using spectrophotometric methods, while 8-hydroxydeoxyguanosine (8-OHdG) and malondialdehyde (MDA) were determined using immunoassays.

No significant differences for salivary OS markers between men and women were observed.

For all examined OS markers, no significant day-to-day variations were demonstrated.

Significant diurnal variations were found in salivary GPX, TAC and MDA levels.

For SOD, TAC, GPX, and UA, good-to-moderate intraindividual coefficients of variations (CVs) were observed in more than 75% of the subjects.

For MDA and 8-OHdG, intraindividual CVs > 35% were observed in 60% and 40% of the subjects, respectively.

Between-subject variance was wide for all examined OS markers (CV% 30.08%–85.70%).

Due to high intraindividual variability in the salivary concentrations of MDA and 8-OHdG, those markers cannot be reliably verified based on single measurements and multiple measurements over several days would provide more reliable information.

Salivary SOD, TAC, GPX, and UA proved stable across three days of measurement.

Trial Registration.

ClinicalTrials.gov NCT03029494.

Registered on 2017-01-19.

American Psychological Association (APA)

Alajbeg, Iva Z.& Lapić, Ivana& Rogić, Dunja& Vuletić, Lea& Andabak Rogulj, Ana& Illeš, Davor…[et al.]. 2017. Within-Subject Reliability and between-Subject Variability of Oxidative Stress Markers in Saliva of Healthy Subjects: A Longitudinal Pilot Study. Disease Markers،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1152253

Modern Language Association (MLA)

Alajbeg, Iva Z.…[et al.]. Within-Subject Reliability and between-Subject Variability of Oxidative Stress Markers in Saliva of Healthy Subjects: A Longitudinal Pilot Study. Disease Markers No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1152253

American Medical Association (AMA)

Alajbeg, Iva Z.& Lapić, Ivana& Rogić, Dunja& Vuletić, Lea& Andabak Rogulj, Ana& Illeš, Davor…[et al.]. Within-Subject Reliability and between-Subject Variability of Oxidative Stress Markers in Saliva of Healthy Subjects: A Longitudinal Pilot Study. Disease Markers. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1152253

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1152253