Allium stipitatum Extract Exhibits In Vivo Antibacterial Activity against Methicillin-Resistant Staphylococcus aureus and Accelerates Burn Wound Healing in a Full-Thickness Murine Burn Model

Abstract EN

The in vivo antibacterial and burn wound healing potency of Persian shallot bulbs (Allium stipitatum) were explored in a mice burn model infected with methicillin-resistant Staphylococcus aureus (MRSA).

Hexane (ASHE) and dichloromethane (ASDE) extracts were tested.

Female BALB/c mice were inflicted with third-degree thermal injury followed by infection with MRSA.

ASHE and ASDE formulated with simple ointment base (SOB) at concentrations of 1%, 2%, and 5% (w/w) were topically applied to burn wounds twice a day for 20 days.

Silver sulfadiazine (1%) served as drug positive control.

Microbiological analysis was carried out on 1, 2, 3, 4, and 5 days postwounding (dpw) and histopathological analysis at the end of the experiment (20 dpw).

Both ointments demonstrated strong antibacterial activity with complete elimination of MRSA at 48–72 h after infection.

The rate of wound contraction was higher (95–100%) in mice groups treated with ASHE and ASDE ointments after 15 dpw.

Histological analysis revealed significant increase ( p < 0.05 ) in epithelialization and collagenation in treated groups.

The ASHE and ASDE were found to be relatively noncytotoxic and safe to Vero cell line (383.4 μg mL−1; 390.6 μg mL−1), suggesting the extracts as safe topical antibacterial as well as promising alternatives in managing thermal injuries.